14-3-3 ε Antibody (YA7622)
(Synonyms: 14-3-3 protein epsilon, 14-3-3E, YWHAE)14-3-3 ε Antibody (YA7622) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to 14-3-3 ε.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
14-3-3 ε Antibody (YA7622) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to 14-3-3 ε.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 29 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 29 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
14-3-3ε is a key member of the 14-3-3 protein family and a widely expressed molecule that binds to phosphorylated proteins, playing a critical role in cell signaling, cell cycle regulation, and neurodevelopment. It serves as a biomarker for Miller-Dieker lissencephaly syndrome, a condition characterized by neuronal migration defects caused by YWHAE gene deletion.
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Subcellular Localization
Nucleus,Cytoplasm,Melanosome
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Isoforms & Post-Translational Modification
14-3-3 ε has two isomers: P62258-1: 29174 Da (predicted); P62258-2: 26504 Da (predicted).
(Microbial infection) Cleaved by poliovirus protease 3C, leading to disruption of the interaction with RIGI -
Subunit
Homodimer
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SwissProt ID
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Synonyms
14-3-3 protein epsilon, 14-3-3E, YWHAE
Documentation
References
[1]. Obsilova V, et al. Structural insights into the functional roles of 14-3-3 proteins. Front Mol Biosci. 2022;9:1016071. [Content Brief]
[2]. Muslin AJ, et al. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine. Cell. 1996;84(6):889-897. [Content Brief]
[3]. Yang X, et al. Structural basis for protein-protein interactions in the 14-3-3 protein family. Proc Natl Acad Sci U S A. 2006;103(46):17237-17242. [Content Brief]
[4]. Gardino AK, et al. 14-3-3 proteins as signaling integration points for cell cycle control and apoptosis. Semin Cell Dev Biol. 2011;22(7):688-695. [Content Brief]
[5]. Pennington KL, et al. The dynamic and stress-adaptive signaling hub of 14-3-3: emerging mechanisms of regulation and context-dependent protein-protein interactions. Oncogene. 2018;37(42):5587-5604. [Content Brief]
[6]. Li Z, et al. 14-3-3σ, the double-edged sword of human cancers. Am J Transl Res. 2009;1(4):326-340. [Content Brief]
[7]. Maksymowych WP, et al. Serum 14-3-3η is a novel marker that complements current serological measurements to enhance detection of patients with rheumatoid arthritis. J Rheumatol. 2014;41(11):2104-2113. [Content Brief]
[8]. Aghazadeh Y, et al. The role of the 14-3-3 protein family in health, disease, and drug development. Drug Discov Today. 2016;21(2):278-287. [Content Brief]
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