14-3-3 ε Antibody (YA7622)

製品番号: HY-P87937: データシート User Guide for Antibodies
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14-3-3 ε Antibody (YA7622) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to 14-3-3 ε.

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容量		在庫状況
50 μL		お問い合わせ
100 μL		お問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

14-3-3 ε Antibody (YA7622) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to 14-3-3 ε.

Host

Rabbit

Clonality

Monoclonal,Recombinant

分子量

Predicted band size: 29 kDa;

Observed band size: 29 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P62258

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	純度	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:21189250). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed:37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed:22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed:37599448)

Subcellular Localization：Nucleus,Cytoplasm,Melanosome

Isoforms & Post-Translational Modification：P62258 has two isomers: P62258-1: 29174 Da (predicted); P62258-2: 26504 Da (predicted).
(Microbial infection) Cleaved by poliovirus protease 3C, leading to disruption of the interaction with RIGI

Subunit：Homodimer (PubMed:17085597)

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/sw-620-cell.html [Content Brief]