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SynGAP Antibody (YA1689)

(Synonyms: MRD5; p135 SynGAP; RASA1; RASA5; SYNGAP1)
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Based on 1 Customer Validation

SynGAP Antibody (YA1689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SynGAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SynGAP Antibody (YA1689)
  • SynGAP Antibody (YA1689) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SynGAP Antibody (YA1689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SynGAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Rat
  • Observed Molecular Weight
    Observed band size: 148 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 148 kDa
Species Reactivity Database

Entrez Gene: 192117 Rat

SwissProt: Q9QUH6 Rat

Immunogen

A synthetic peptide of human SynGAP aa1330-1343.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104065

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SynGAP Antibody (YA1689)
    Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using SynGAP Antibody (HY-P81944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SynGAP is a Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. Exhibits dual GTPase-activating specificity for Ras and Rap. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits

  • Isoforms & Post-Translational Modification

    Q96PV0 has 4 isomers: Q96PV0-1: 148284 Da (predicted); Q96PV0-2: 141248 Da (predicted); Q96PV0-3: 140929 Da (predicted); Q96PV0-4: 143569 Da (predicted).
    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Phosphorylation by PLK2 promotes its activity (By similarity)

  • Subunit

    Interacts with MPDZ (PubMed:15312654).

  • SwissProt ID

    Q96PV0

  • Gene ID
    8831 [NCBI]
  • Synonyms

    MRD5; p135 SynGAP; RASA1; RASA5; SYNGAP1

  • Research Field

    Neuroscience

SynGAP Antibody (YA1689) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using SynGAP Antibody (HY-P81944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SynGAP Antibody (YA1689) Product Image
    1/16
  • Experimental Validation Results for SynGAP Antibody (YA1689)
  • SynGAP Antibody (YA1689) Product Image
  • Experimental Validation Results for SynGAP Antibody (YA1689)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using SynGAP Antibody (HY-P81944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SynGAP Antibody (YA1689)
  • SynGAP Antibody (YA1689) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using SynGAP Antibody (HY-P81944) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SynGAP Antibody (YA1689) Product Image WB