PIAS3 Antibody
(Synonyms: E3 SUMO-protein ligase PIAS3, E3 SUMO-protein transferase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3)
Based on 1 Customer Validation
PIAS3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PIAS3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat, Pig, Dog, Bovine
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Formulation:
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:100-200 |
Product Details
PIAS3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PIAS3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat, Pig, Dog, Bovine
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
Synthetic peptide corresponding to the N-term region of human PIAS3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIAS3 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus[1][2][3][4].
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Subcellular Localization
Cytoplasm; Nucleus; Nucleus speckle
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Expression
Tissue_Specificity: Widely expressed.
Induction: By dihydrotestosterone (DHT) in prostate cancer cells. -
Isoforms & Post-Translational Modification
PIAS3 has an amino acid length of 628, molecular weight is 68017 Da.
Sumoylated. -
Subunit
Monomer (By similarity).
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SwissProt ID
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Synonyms
E3 SUMO-protein ligase PIAS3, E3 SUMO-protein transferase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3
Documentation
[1]. Cong L, et al. SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function. J Biol Chem. 2011 Dec 23;286(51):43793-43808. [Content Brief]
[2]. Chung CD, et al. Specific inhibition of Stat3 signal transduction by PIAS3. Science. 1997 Dec 5;278(5344):1803-5. [Content Brief]
[3]. Park JH, et al. Modification of DBC1 by SUMO2/3 is crucial for p53-mediated apoptosis in response to DNA damage. Nat Commun. 2014 Nov 18;5:5483. [Content Brief]
[4]. Sun X, et al. Characterization of nuclear localization and SUMOylation of the ATBF1 transcription factor in epithelial cells. PLoS One. 2014;9(3):e92746. [Content Brief]
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