LIM Kinase 1 Antibody (YA9710)
(Synonyms: EC 2.7.1.37, LIM domain containing protein kinase, LIM domain kinase 1, LIM kinase, LIM motif containing protein kinase, LIMK 1, LIMK, LIMK-1, limk1, LIMK1_HUMAN)LIM Kinase 1 Antibody (YA9710) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to LIM Kinase 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:4000 | 1:200-1000 |
Product Details
LIM Kinase 1 Antibody (YA9710) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to LIM Kinase 1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat, Monkey
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Calculated Molecular Weight Predicted band size: 73 kDa;
Recombinant protein of human (aa 1-350).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
LIM Kinase 1 is a Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development; Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1)[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cytoplasm; Nucleus; Cytoplasm, cytoskeleton; Cell projection, lamellipodium
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Expression
Tissue_specificity:It is expressed at the highest levels throughout the human nervous system. It is widely expressed in all regions of the human brain, with the highest expression level in the cerebral cortex. Expression levels are lower in the heart and skeletal muscle. -
Isoforms & Post-Translational Modification
P53667 has 4 isomers: P53667-1: 72585 Da (predicted); P53667-2: 70793 Da (predicted); P53667-3: 33373 Da (predicted); P53667-4: 68729 Da (predicted).
Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP;Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity) -
Subunit
Interacts (via LIM domain) with the cytoplasmic domain of NRG1. Interacts with NISCH. Interacts with RLIM and RNF6 (By similarity). Self-associates to form homodimers (PubMed:10196227). Interacts with HSP90AA1; this interaction promotes LIMK1 dimerization and subsequent transphosphorylation (PubMed:16641196). Interacts with CDKN1C (PubMed:14530263). Interacts with SSH1 (PubMed:15660133). Interacts with ROCK1 (PubMed:10436159, PubMed:10652353). Interacts (via LIM zinc-binding domains) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with CDC42BPA, CDC42BPB and FAM89B/LRAP25 (By similarity)
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SwissProt ID
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Synonyms
EC 2.7.1.37, LIM domain containing protein kinase, LIM domain kinase 1, LIM kinase, LIM motif containing protein kinase, LIMK 1, LIMK, LIMK-1, limk1, LIMK1_HUMAN
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