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NALP2 Antibody (YA8930)

(Synonyms: NALP2, NBS1, PAN1, PYPAF2, NLRP2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2)
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Based on 1 Customer Validation

NALP2 Antibody (YA8930) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NALP2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human, mouse, rat

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NALP2 Antibody (YA8930)
  • NALP2 Antibody (YA8930) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:30-3000

Product Details

Description

NALP2 Antibody (YA8930) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NALP2.

  • Host Mouse
  • Species Reactivity
    human, mouse, rat
  • Calculated Molecular Weight Predicted band size: 117 kDa
Species Reactivity Database

OMIM: 609364

Immunogen

A synthesized peptide derived from human NALP2.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NALP2 Antibody (YA8930)
    Western blot analysis was performed on protein extracts (25 μg) from K562 (lane 2) and HeLa (lane 3) using NALP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    NALP2 suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature pro-inflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of pro-inflammatory caspases

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain. Highly abundant in oocytes and early embryos, however poorly expressed in somatic tissues such as brain, kidney, liver and spinal cord.
    Induction: By interferons and bacterial lipopolysaccharides (LPS).

  • Isoforms & Post-Translational Modification

    NALP2 has 5 isoforms, Q9NX02-1: amino acid length is 1062, molecular weight is 120515 Da (predicted); Q9NX02-2: amino acid length is 1040, molecular weight is 118138 Da (predicted); Q9NX02-3: amino acid length is 846, molecular weight is 96383 Da (predicted); Q9NX02-4: amino acid length is 1038, molecular weight is 117674 Da (predicted); Q9NX02-5: amino acid length is 1039, molecular weight is 117883 Da (predicted).

  • Subunit

    Interacts with CHUK.

  • SwissProt ID

    Q9NX02

  • Gene ID
    55655 [NCBI]
  • Synonyms

    NALP2, NBS1, PAN1, PYPAF2, NLRP2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2

NALP2 Antibody (YA8930) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from K562 (lane 2) and HeLa (lane 3) using NALP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NALP2 Antibody (YA8930) Product Image
    1/16
  • Experimental Validation Results for NALP2 Antibody (YA8930)
  • NALP2 Antibody (YA8930) Product Image
  • Experimental Validation Results for NALP2 Antibody (YA8930)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from K562 (lane 2) and HeLa (lane 3) using NALP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NALP2 Antibody (YA8930)
  • NALP2 Antibody (YA8930) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from K562 (lane 2) and HeLa (lane 3) using NALP2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NALP2 Antibody (YA8930) Product Image WB