TEAD2 Antibody (YA6796)

Cat. No.: HY-P87103: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TEAD2 Antibody (YA6796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TEAD2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

TEAD2 Antibody (YA6796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TEAD2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q15562

Gene ID

8463 [NCBI]

Immunogen

Recombinant protein within human TEAD2 aa 101-300.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2, Negative), HeLa (lane 3), 293T (lane 4), A549 (lane 5), MCF-7 (lane 6), and HepG2 (lane 7) using TEAD2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：Q15562 has four isomers: Q15562-1: 49243 Da (predicted); Q15562-2: 49569 Da (predicted); Q15562-3: 35403 Da (predicted); Q15562-4: 49640 Da (predicted).

Subunit：Interacts with YAP1 and WWTR1/TAZ

Synonyms

Embryonic TEA domain containing factor antibody; Etdf antibody; ETEF 1 antibody; ETF antibody; TEA domain family member 2 antibody; TEAD 2 antibody; TEAD-2 antibody; TEAD2 antibody; Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody; TEAD2_HUMAN antibody; Embryonic TEA domain containing factor antibody; Etdf antibody; ETEF 1 antibody; ETF antibody; TEA domain family member 2 antibody; TEAD 2 antibody; TEAD-2 antibody; TEAD2 antibody; Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody; TEAD2_HUMAN antibody; TEF 4 antibody; TEF4 antibody; Transcriptional enhancer factor 4 antibody; Transcriptional enhancer factor TEF 4 antibody; Transcriptional enhancer factor TEF-4 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (253 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)