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DNAJB6 Antibody (YA2704)

(Synonyms: DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E)
Customer Review

Based on 1 Customer Validation

DNAJB6 Antibody (YA2704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJB6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • DNAJB6 Antibody (YA2704) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

DNAJB6 Antibody (YA2704) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJB6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human DNAJB6 aa162-175.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), MDA-MB-231 (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using DNAJB6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    DNAJB6 is a Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Also reduces cellular toxicity and caspase-3 activity; Isoform B but not isoform A inhibits huntingtin aggregation[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, perinuclear region; Nucleus; Cytoplasm, myofibril, sarcomere, Z line

  • Expression


    Tissue_specificity:Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus

  • Isoforms & Post-Translational Modification

    O75190 has 4 isomers: O75190-1: 36087 Da (predicted); O75190-2: 26900 Da (predicted); O75190-3: 35714 Da (predicted); O75190-4: 30369 Da (predicted).

  • Subunit

    Homooligomer (PubMed:22366786). Interacts with BAG3, HSPB8 and STUB1 (PubMed:22366786). Interacts with ALKBH1 (By similarity). Interacts with HSP70, KRT18 and PTTG (PubMed:10954706, PubMed:9915854)

  • SwissProt ID

    O75190

  • Gene ID
    10049 [NCBI]
  • Synonyms

    DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E

  • Research Field

    Neuroscience

DNAJB6 Antibody (YA2704) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), MDA-MB-231 (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using DNAJB6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    1/16
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • DNAJB6 Antibody (YA2704) Product Image
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), MDA-MB-231 (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using DNAJB6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • Experimental Validation Results for DNAJB6 Antibody (YA2704)
  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), MDA-MB-231 (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using DNAJB6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung Adenocarcinom tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer‌ tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using DNAJB6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P82959, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical cancer tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis‌ tissue using DNAJB6 A antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P82959, 1:200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • DNAJB6 Antibody (YA2704) Product Image WB
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image IHC-P
  • DNAJB6 Antibody (YA2704) Product Image mIHC
  • DNAJB6 Antibody (YA2704) Product Image mIHC
  • DNAJB6 Antibody (YA2704) Product Image mIHC
  • DNAJB6 Antibody (YA2704) Product Image mIHC
  • DNAJB6 Antibody (YA2704) Product Image mIHC
  • DNAJB6 Antibody (YA2704) Product Image mIHC