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PPAR alpha Antibody (YA6141)

(Synonyms: PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1)
Customer Review

Based on 1 Customer Validation

PPAR alpha Antibody (YA6141) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPAR alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PPAR alpha Antibody (YA6141)
  • PPAR alpha Antibody (YA6141) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:5000-1:20000 1:50-1:200

Product Details

Description

PPAR alpha Antibody (YA6141) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPAR alpha.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719161

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PPAR alpha Antibody (YA6141)
    Western blot analysis of extracts from HepG2(lane 2(20μg), NIH-3T3(lane 3(20μg), MCF-7(lane 4(20μg) and Hela(lane 5(20μg) using PPAR alpha Antibody（HY-P86449）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PPAR alpha is a Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Skeletal muscle, liver, heart, and kidneys. Expressed in monocytes (PubMed: 28167758) .

    Induction:Down-regulated by aging

  • Isoforms & Post-Translational Modification

    Q07869 has 2 isomers: Q07869-1: 52225 Da (predicted); Q07869-2: 18942 Da (predicted).
    Ubiquitinated by E3 ubiquitin-protein ligase HUWE1; leading to proteasomal degradation;Phosphorylated

  • Subunit

    Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with NCOA3 coactivator. Interacts with CITED2; the interaction stimulates its transcriptional activity.

  • SwissProt ID

    Q07869

  • Gene ID
    5465 [NCBI]
  • Synonyms

    PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1

PPAR alpha Antibody (YA6141) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20μg), NIH-3T3(lane 3(20μg), MCF-7(lane 4(20μg) and Hela(lane 5(20μg) using PPAR alpha Antibody（HY-P86449）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PPAR alpha Antibody (YA6141) Product Image
    1/16
  • Experimental Validation Results for PPAR alpha Antibody (YA6141)
  • PPAR alpha Antibody (YA6141) Product Image
  • Experimental Validation Results for PPAR alpha Antibody (YA6141)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20μg), NIH-3T3(lane 3(20μg), MCF-7(lane 4(20μg) and Hela(lane 5(20μg) using PPAR alpha Antibody（HY-P86449）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PPAR alpha Antibody (YA6141)
  • PPAR alpha Antibody (YA6141) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20μg), NIH-3T3(lane 3(20μg), MCF-7(lane 4(20μg) and Hela(lane 5(20μg) using PPAR alpha Antibody（HY-P86449）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PPAR alpha Antibody (YA6141) Product Image WB