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ATP Citrate Lyase Antibody (YA829)(PBS only)

(Synonyms: ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme)

ATP Citrate Lyase Antibody (YA829) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP Citrate Lyase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP Citrate Lyase Antibody (YA829)(PBS only)
  • ATP Citrate Lyase Antibody (YA829)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

ATP Citrate Lyase Antibody (YA829) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP Citrate Lyase.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 121 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 121 kDa
Immunogen

Synthetic peptide corresponding to Human ATP-Citrate Lyase.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP Citrate Lyase Antibody (YA829)(PBS only)
    Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ATP-citrate lyase (ACLY) is a central metabolic enzyme and catalyses the ATP-dependent conversion of citrate and coenzyme A (CoA) to oxaloacetate and acetyl-CoA. The acetyl-CoA product is crucial for the metabolism of fatty acids, the biosynthesis of cholesterol, and the acetylation and prenylation of proteins. Acetyl CoA is also required for acetylation reactions that modify proteins, such as histone acetylation. ACLY is upregulated or activated in several types of cancers, and its inhibition is known to induce proliferation arrest in cancer cells both in vitro and in vivo.

    ACLY links glucose and lipid metabolism by catalyzing the formation of acetyl-CoA and oxaloacetate from citrate produced by glycolysis in the presence of ATP and CoA. ACLY is aberrantly expressed in many immortalized cells and tumors, such as breast, liver, colon, lung and prostate cancers, and is correlated reversely with tumor stage and differentiation, serving as a negative prognostic marker. ACLY is an upstream enzyme of the long chain fatty acid synthesis, providing acetyl-CoA as an essential component of the fatty acid synthesis. Therefore, ACLY is a key enzyme of cellular lipogenesis and potent target for cancer therapy.

  • Subcellular Localization

    Cytoplasm, cytosol

  • Isoforms & Post-Translational Modification

    P53396 has 3 isomers: P53396-1: 120839 Da (predicted); P53396-2: 119772 Da (predicted); P53396-3: 91099 Da (predicted).
    Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665). Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity). Phosphorylated at Ser-455 by BCKDK and dephosphorylated by protein phosphatase PPM1K;ISGylated;Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781). Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781). Acetylation promotes de novo lipid synthesis (PubMed:23932781). Deacetylated by SIRT2;Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895). Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895)

  • Subunit

    Homotetramer

  • SwissProt ID

    P53396

  • Gene ID
    47 [NCBI]
  • Synonyms

    ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme

  • Research Field

    Signal Transduction

ATP Citrate Lyase Antibody (YA829)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • ATP Citrate Lyase Antibody (YA829)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ATP Citrate Lyase Antibody (YA829)(PBS only)
  • ATP Citrate Lyase Antibody (YA829)(PBS only) Product Image
  • Experimental Validation Results for ATP Citrate Lyase Antibody (YA829)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP Citrate Lyase Antibody (YA829)(PBS only)
  • ATP Citrate Lyase Antibody (YA829)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • ATP Citrate Lyase Antibody (YA829)(PBS only) Product Image WB