ATP Citrate Lyase Antibody (YA829)

Cat. No.: HY-P80560: Data Sheet COA
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ATP Citrate Lyase Antibody (YA829) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP Citrate Lyase.

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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ATP Citrate Lyase Antibody (YA829) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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ATP Citrate Lyase Antibody (YA829) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP Citrate Lyase.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 121 kDa;

Observed band size: 121 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

P53396

Gene ID

47 [NCBI]

Immunogen

Synthetic peptide corresponding to Human ATP-Citrate Lyase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism

Subcellular Localization：Cytoplasm, cytosol

Isoforms & Post-Translational Modification：P53396 has 3 isomers: P53396-1: 120839 Da (predicted); P53396-2: 119772 Da (predicted); P53396-3: 91099 Da (predicted).
Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665). Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity). Phosphorylated at Ser-455 by BCKDK and dephosphorylated by protein phosphatase PPM1K;ISGylated;Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781). Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781). Acetylation promotes de novo lipid synthesis (PubMed:23932781). Deacetylated by SIRT2;Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895). Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895)

Subunit：Homotetramer

RRID

AB_3102130

Database

Entrez Gene: 47 Human ; 104112 Mouse ;

SwissProt: P53396 Human ; Q91V92 Mouse ;

OMIM: 108728 Human

Research Field

Signal Transduction

Synonyms

ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

ATP Citrate Lyase Antibody (YA829) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ATP Citrate Lyase Antibody (YA829)

ATP Citrate Lyase Antibody (YA829) Featured Recommendations:

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ATP Citrate Lyase Antibody (YA829) Related Classifications

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