Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646)

Cat. No.: HY-P80482: Data Sheet COA
SDS
User Guide for Antibodies
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Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl CoA Carboxylase 1 (ACC1).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) Featured Recommendations:

1 Publications Citing Use of MCE Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646)

•Drug Metab Dispos. 2025 Dec 4;54(1):100211. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl CoA Carboxylase 1 (ACC1).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 266 kDa;

Observed band size: 250-300 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q13085

Gene ID

31 [NCBI]

Immunogen

Synthetic peptide corresponding to Human ACC1.AA range:382-427.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of extracts from Hela(lane2(20μg) , HepG2(lane 3(20μg) and C6(lane 4(20ug) using Acetyl CoA Carboxylase 1 Antibody (HY-P80482) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Acetyl CoA Carboxylase 1 (ACC1) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80482, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Acetyl CoA Carboxylase 1 (ACC1) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80482, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:It is expressed in the brain, placenta, skeletal muscle, kidney, pancreas, and adipose tissue; it is expressed at low levels in lung tissue; and it was not detected in the liver.

Subunit：Monomer, homodimer, and homotetramer (PubMed:20952656, PubMed:29899443). Can form filamentous polymers (PubMed:20457939, PubMed:20952656, PubMed:29899443). Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis (PubMed:12360400, PubMed:16326698, PubMed:18452305, PubMed:29899443). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity (PubMed:20457939)

RRID

AB_3102555

Database

Entrez Gene: 31 Human ; 32 Human ; 100705 Mouse ; 107476 Mouse ;

SwissProt: Q13085 Human ; Q5SWU9 Mouse ; P11497 Rat

OMIM: 613933 Human

Research Field

Cardiovascular

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646)

Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) Featured Recommendations:

1 Publications Citing Use of MCE Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646)

Product Detail

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Background

Documentation

Acetyl CoA Carboxylase 1 (ACC1) Antibody (YA646) Related Classifications

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