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TERT Antibody (YA3439)

(Synonyms: Telomerase catalytic subunit; EST2; hEST2; TCS1; Telomerase associated protein 2; Telomere Reverse Transcriptase; TP2; TRT; Telomerase reverse transcriptase; Telomerase Catalytic Subunit; Telomerase-associated protein 2; TERT_HUMAN.)
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Based on 1 Customer Validation

TERT Antibody (YA3439) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TERT.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

WB
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • TERT Antibody (YA3439) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TERT Antibody (YA3439) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TERT.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 127 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Species Reactivity Database

Entrez Gene: 7015 Human

SwissProt: O14746 Human

Immunogen

A synthesized peptide derived from human TERT aa1000-1132.

Sensitivity

Endogenous

Purification

affinity purified

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TERT Antibody (YA3439)
    Western blot analysis of extracts from K562 using TERT antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for TERT Antibody (YA3439)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), HT-29 (lane 3, 15 μg), PC-3 (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Telomerase reverse transcriptase Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TERT Antibody (YA3439)
    Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg), PC-3 (lane 4(20μg), HT-29 (lane 5(20μg) using TERT Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TERT is a Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis

  • Subcellular Localization

    Nucleus, nucleolus; Nucleus, nucleoplasm; Nucleus; Chromosome, telomere; Cytoplasm; Nucleus, PML body

  • Expression


    Tissue_specificity:It is highly expressed in thymocyte subsets, moderately expressed in tonsillar T lymphocytes, and lowly expressed or undetectable in peripheral blood T lymphocytes.

    Induction:Activated by cytotoxic events and down-regulated during aging. In peripheral T-lymphocytes, induced By CD3 and by PMA/ionomycin. Inhibited by herbimycin B

  • Isoforms & Post-Translational Modification

    O14746 has 4 isomers: O14746-1: 126997 Da (predicted); O14746-2: 90226 Da (predicted); O14746-3: 120048 Da (predicted); O14746-4: 88965 Da (predicted).
    Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation at Ser-227 by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-457 by DYRK2 promotes ubiquitination by the EDVP complex and degradation;Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-457 by DYRK2. Ubiquitinated leads to proteasomal degradation;(Microbial infection) In case of infection by HIV-1, the EDVP complex is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP, leading to ubiquitination and degradation

  • Subunit

    Catalytic component of the telomerase holoenzyme complex composed of one molecule of TERT, one molecule of WRAP53/TCAB1, two molecules of H/ACA ribonucleoprotein complex subunits DKC1, NOP10, NHP2 and GAR1, and a telomerase RNA template component (TERC) (PubMed:19179534, PubMed:20351177, PubMed:29695869).

  • SwissProt ID

    O14746

  • Gene ID
    7015 [NCBI]
  • Synonyms

    Telomerase catalytic subunit; EST2; hEST2; TCS1; Telomerase associated protein 2; Telomere Reverse Transcriptase; TP2; TRT; Telomerase reverse transcriptase; Telomerase Catalytic Subunit; Telomerase-associated protein 2; TERT_HUMAN.

  • Research Field

    Epigenetics and Nuclear Signaling

TERT Antibody (YA3439) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 using TERT antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), HT-29 (lane 3, 15 μg), PC-3 (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Telomerase reverse transcriptase Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg), PC-3 (lane 4(20μg), HT-29 (lane 5(20μg) using TERT Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TERT Antibody (YA3439) Product Image
    1/16
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • TERT Antibody (YA3439) Product Image
  • Experimental Validation Results for TERT Antibody (YA3439)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 using TERT antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for TERT Antibody (YA3439)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), HT-29 (lane 3, 15 μg), PC-3 (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Telomerase reverse transcriptase Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TERT Antibody (YA3439)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg), PC-3 (lane 4(20μg), HT-29 (lane 5(20μg) using TERT Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • Experimental Validation Results for TERT Antibody (YA3439)
  • TERT Antibody (YA3439) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 using TERT antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • TERT Antibody (YA3439) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), HT-29 (lane 3, 15 μg), PC-3 (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Telomerase reverse transcriptase Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TERT Antibody (YA3439) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg), PC-3 (lane 4(20μg), HT-29 (lane 5(20μg) using TERT Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TERT Antibody (YA3439) Product Image WB
  • TERT Antibody (YA3439) Product Image WB
  • TERT Antibody (YA3439) Product Image WB