ARA70 Antibody (YA7437)
ARA70 Antibody (YA7437) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ARA70.
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Host:
Rabbit
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Isotype:
IgG, Kappa
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Application:
WB, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
ARA70 Antibody (YA7437) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ARA70.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 75,76 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 70 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG, Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ARA70 is a Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis. Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy. Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion. Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation. In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR. Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function. May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasmic vesicle, autophagosome,Autolysosome,Nucleus,Chromosome
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Expression
Tissue_Specificity: Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis
Induction: Down-regulated by nutrient deprivation (at protein level) -
Isoforms & Post-Translational Modification
Q13772 has four isomers: Q13772-1: 69726 Da (predicted); Q13772-2: 32562 Da (predicted); Q13772-3: 71371 Da (predicted); Q13772-4: 73649 Da (predicted).
NCOA4 levels may be regulated via HERC2-mediated ubiquitination leading to proteosomal degradation; HERC2 association is dependent on NCOA4 iron occupancy -
Subunit
Interacts with the ferritin complex, an oligomeric structure composed of varying combinations of ferritin heavy chains and ferritin light chains (PubMed:25327288)
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SwissProt ID
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