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CD11b Antibody (YA546)

(別名: CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; CD11b; Leukocyte adhesion receptor MO1)
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CD11b Antibody (YA546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD11b Antibody (YA546)
  • CD11b Antibody (YA546) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

MedChemExpress（MCE）の使用を引用している文献 CD11b Antibody (YA546)

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

製品説明

CD11b Antibody (YA546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 170 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Species Reactivity Database

Entrez Gene: 16409 Mouse

SwissProt: P05555 Mouse

Immunogen

Synthetic peptide corresponding to Mouse CD11b.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102620

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • 輸送条件

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD11b Antibody (YA546)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) , TF-1(lane 3(20ug) and J774A.1(lane 4(20ug) using CD11b Antibody (HY-P80592) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed:18685038)

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Predominantly expressed in monocytes and granulocytes (PubMed:3887182, PubMed:8986723) . Expressed in a subset of peritoneal mast cells (PubMed:9862668) . Expressed in microglia (at protein level) (PubMed:18685038)

    Induction:Induced by lipopolysaccharide (LPS) in mast cells

  • Isoforms & Post-Translational Modification

    P05555 has 2 isomers: P05555-1: 127481 Da (predicted); P05555-2: 115196 Da (predicted).

  • Subunit

    Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD. Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance (By similarity). Interacts with TMEM268; this interaction inhibits ITGAM degradation via the endosome-lysosome pathway (By similarity)

  • SwissProt ID

    P05555

  • 遺伝子ID
    16409 [NCBI]
  • 別名

    CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; CD11b; Leukocyte adhesion receptor MO1

  • Research Field

    Neuroscience

CD11b Antibody (YA546) 関連分類

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) , TF-1(lane 3(20ug) and J774A.1(lane 4(20ug) using CD11b Antibody (HY-P80592) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD11b Antibody (YA546) Product Image
    1/16
  • Experimental Validation Results for CD11b Antibody (YA546)
  • CD11b Antibody (YA546) Product Image
  • Experimental Validation Results for CD11b Antibody (YA546)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) , TF-1(lane 3(20ug) and J774A.1(lane 4(20ug) using CD11b Antibody (HY-P80592) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD11b Antibody (YA546)
  • CD11b Antibody (YA546) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) , TF-1(lane 3(20ug) and J774A.1(lane 4(20ug) using CD11b Antibody (HY-P80592) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD11b Antibody (YA546) Product Image WB