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Phospho-PKA R2/PKR2 (Ser99) Antibody

(Synonyms: PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Phospho-PKA R2/PKR2 (Ser99) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PKA R2/PKR2 (Ser99).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat, Pig

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-PKA R2/PKR2 (Ser99) Antibody
  • Phospho-PKA R2/PKR2 (Ser99) Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:20

Product Details

Description

Phospho-PKA R2/PKR2 (Ser99) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PKA R2/PKR2 (Ser99).

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat, Pig
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser99 of Human Phospho-PKA R2.AA range:436-485.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102230

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-PKA R2/PKR2 (Ser99) Antibody
    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-PKA RII alpha Antibody (HY-P80847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PKA R2/PKR2 is a Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:There are four known types of regulatory chains: I-α, I-β, II-α, and II-β. Their expression varies in different tissues; some are constitutively expressed, while others are inducibly expressed.

  • Isoforms & Post-Translational Modification

    P13861 has 2 isomers: P13861-1: 45518 Da (predicted); P13861-2: 43067 Da (predicted).
    Phosphorylated by the activated catalytic chain

  • Subunit

    The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains.

  • SwissProt ID

    P13861

  • Gene ID
    5576 [NCBI]
  • Synonyms

    PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit

  • Research Field

    Signal Transduction

Phospho-PKA R2/PKR2 (Ser99) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-PKA RII alpha Antibody (HY-P80847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-PKA R2/PKR2 (Ser99) Antibody Product Image
    1/16
  • Experimental Validation Results for Phospho-PKA R2/PKR2 (Ser99) Antibody
  • Phospho-PKA R2/PKR2 (Ser99) Antibody Product Image
  • Experimental Validation Results for Phospho-PKA R2/PKR2 (Ser99) Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-PKA RII alpha Antibody (HY-P80847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-PKA R2/PKR2 (Ser99) Antibody
  • Phospho-PKA R2/PKR2 (Ser99) Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-PKA RII alpha Antibody (HY-P80847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-PKA R2/PKR2 (Ser99) Antibody Product Image WB