Phospho-PKA R2/PKR2 (Ser99) Antibody

Cat. No.: HY-P80847: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-PKA R2/PKR2 (Ser99) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PKA R2/PKR2 (Ser99).

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-PKA R2/PKR2 (Ser99) Antibody Featured Recommendations:

2 Publications Citing Use of MCE Phospho-PKA R2/PKR2 (Ser99) Antibody

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Phospho-PKA R2/PKR2 (Ser99) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PKA R2/PKR2 (Ser99).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Pig

SwissProt ID

P13861

Gene ID

5576 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser99 of Human Phospho-PKA R2.AA range:436-485.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using Phospho-PKA RII alpha Antibody (HY-P80847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:There are four known types of regulatory chains: I-α, I-β, II-α, and II-β. Their expression varies in different tissues; some are constitutively expressed, while others are inducibly expressed.

Isoforms & Post-Translational Modification：P13861 has 2 isomers: P13861-1: 45518 Da (predicted); P13861-2: 43067 Da (predicted).
Phosphorylated by the activated catalytic chain

Subunit：The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains.

RRID

AB_3102230

Database

Entrez Gene: 5576 Human ; 29699 Rat

SwissProt: P13861 Human ; P12367 Mouse ; P12368 Rat

OMIM: 176910 Human

Research Field

Signal Transduction

Synonyms

PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-PKA R2/PKR2 (Ser99) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-PKA R2/PKR2 (Ser99) Antibody

Phospho-PKA R2/PKR2 (Ser99) Antibody Featured Recommendations:

2 Publications Citing Use of MCE Phospho-PKA R2/PKR2 (Ser99) Antibody

Product Detail

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Background

Documentation

Phospho-PKA R2/PKR2 (Ser99) Antibody Related Classifications

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