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PSMA4 Antibody (YA1765)

(Synonyms: proteasome subunit alpha 4; HC9; PSC9; HsT17706)
Customer Review

Based on 1 Customer Validation

PSMA4 Antibody (YA1765) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSMA4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • PSMA4 Antibody (YA1765) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

PSMA4 Antibody (YA1765) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSMA4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 29 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PSMA4

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104141

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Western blot analysis of extracts from Jurkat (lane2, 20μg), K562 (lane3, 20μg), Hela (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using PSMA4 Antibody (HY-P82020). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    PSMA4 component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex)

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Induction:Down-regulated by antioxidants BO-653 and probucol

  • Isoforms & Post-Translational Modification

    P25789 has 2 isomers: P25789-1: 29484 Da (predicted); P25789-2: 21365 Da (predicted).

  • Subunit

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7

  • SwissProt ID

    P25789

  • Gene ID
    5685 [NCBI]
  • Synonyms

    proteasome subunit alpha 4; HC9; PSC9; HsT17706

  • Research Field

    Cell Biology

PSMA4 Antibody (YA1765) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), K562 (lane3, 20μg), Hela (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using PSMA4 Antibody (HY-P82020). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • PSMA4 Antibody (YA1765) Product Image
    1/16
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • PSMA4 Antibody (YA1765) Product Image
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), K562 (lane3, 20μg), Hela (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using PSMA4 Antibody (HY-P82020). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • Experimental Validation Results for PSMA4 Antibody (YA1765)
  • PSMA4 Antibody (YA1765) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane2, 20μg), K562 (lane3, 20μg), Hela (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using PSMA4 Antibody (HY-P82020). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • PSMA4 Antibody (YA1765) Product Image
    MedChemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human thyroid cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • PSMA4 Antibody (YA1765) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using PSMA4 Antibody (YA1765). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82020, 1/1000) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • PSMA4 Antibody (YA1765) Product Image WB
  • PSMA4 Antibody (YA1765) Product Image IHC-P
  • PSMA4 Antibody (YA1765) Product Image IHC-P
  • PSMA4 Antibody (YA1765) Product Image IHC-P
  • PSMA4 Antibody (YA1765) Product Image mIHC
  • PSMA4 Antibody (YA1765) Product Image mIHC
  • PSMA4 Antibody (YA1765) Product Image mIHC
  • PSMA4 Antibody (YA1765) Product Image mIHC