Triosephosphate Isomerase Antibody (YA2066)

製品番号: HY-P82321: データシート SDS COA User Guide for Antibodies
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Triosephosphate Isomerase Antibody (YA2066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Triosephosphate Isomerase.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Triosephosphate Isomerase Antibody (YA2066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Triosephosphate Isomerase.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 27 kDa;

Observed band size: 27 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P60174

Gene ID

7167 [NCBI]

Immunogen

A synthesized peptide derived from human Triosephosphate isomerase aa200-249/249.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(40μg),A549 (lane 3(40μg), NIH/3T3(lane 4(40μg),C6 (lane 5(40μg)using Triosephosphate Isomerase Antibody (YA2066) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis; It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids

Subcellular Localization：Cytoplasm

Isoforms & Post-Translational Modification：P60174 has 3 isomers: P60174-1: 26669 Da (predicted); P60174-3: 30791 Da (predicted); P60174-4: 17958 Da (predicted).

Subunit：Homodimer

Database

Entrez Gene: 7167 Human ; 21991 Mouse ; 24849 Rat

SwissProt: P60174 Human ; P17751 Mouse ; P48500 Rat

OMIM: 615512 Human

Research Field

Signal Transduction

Synonyms

HEL-S-49; TIM; TPI1; TPID

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)