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Triosephosphate Isomerase Antibody (YA2066)

(Synonyms: HEL-S-49; TIM; TPI1; TPID)
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Based on 1 Customer Validation

Triosephosphate Isomerase Antibody (YA2066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Triosephosphate Isomerase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Triosephosphate Isomerase Antibody (YA2066)
  • Triosephosphate Isomerase Antibody (YA2066) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

Triosephosphate Isomerase Antibody (YA2066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Triosephosphate Isomerase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Triosephosphate isomerase aa200-249/249.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Triosephosphate Isomerase Antibody (YA2066)
    Western blot analysis of extracts from Hela (lane 2(40μg),A549 (lane 3(40μg), NIH/3T3(lane 4(40μg),C6 (lane 5(40μg)using Triosephosphate Isomerase Antibody (YA2066) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Triosephosphate Isomerase is a Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis; It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    P60174 has 3 isomers: P60174-1: 26669 Da (predicted); P60174-3: 30791 Da (predicted); P60174-4: 17958 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P60174

  • Gene ID
    7167 [NCBI]
  • Synonyms

    HEL-S-49; TIM; TPI1; TPID

  • Research Field

    Signal Transduction

Triosephosphate Isomerase Antibody (YA2066) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(40μg),A549 (lane 3(40μg), NIH/3T3(lane 4(40μg),C6 (lane 5(40μg)using Triosephosphate Isomerase Antibody (YA2066) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Triosephosphate Isomerase Antibody (YA2066) Product Image
    1/16
  • Experimental Validation Results for Triosephosphate Isomerase Antibody (YA2066)
  • Triosephosphate Isomerase Antibody (YA2066) Product Image
  • Experimental Validation Results for Triosephosphate Isomerase Antibody (YA2066)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(40μg),A549 (lane 3(40μg), NIH/3T3(lane 4(40μg),C6 (lane 5(40μg)using Triosephosphate Isomerase Antibody (YA2066) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Triosephosphate Isomerase Antibody (YA2066)
  • Triosephosphate Isomerase Antibody (YA2066) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(40μg),A549 (lane 3(40μg), NIH/3T3(lane 4(40μg),C6 (lane 5(40μg)using Triosephosphate Isomerase Antibody (YA2066) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Triosephosphate Isomerase Antibody (YA2066) Product Image WB