IRG1 Antibody (YA9713)
(Synonyms: ACOD1, Aconitate decarboxylase 1, Aconitate decarboxylase, CAD, Cis aconitate decarboxylase, Immune responsive gene 1 protein homolog, Immune-responsive gene 1 protein homolog, Immunoresponsive 1 homolog (mouse), Irg1, IRG1_HUMAN)IRG1 Antibody (YA9713) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to IRG1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH7.4), 50% Glycerol, 0.02% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:5000-50000 | 1:250-1000 |
Product Details
IRG1 Antibody (YA9713) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to IRG1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 54 kDa;
Recombinant protein of mouse IRG1 (aa 280-480).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 50% Glycerol, 0.02% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IRG1 is a Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity)[1][2][3][4].
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Subcellular Localization
Mitochondrion
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Expression
Tissue_Specificity: Expressed in LPS-tolerized macrophages (at protein level). Expressed in peripheral blood mononuclear cells (PBMCs), microglia and macrophage cells.
Induction: Up-regulated after lipopolysaccharide (LPS) stimulation. Up-regulated in LPS-tolerized macrophage by LPS. Up-regulated in peripheral blood mononuclear cells (PBMC) of patient after acute sepsis. -
Isoforms & Post-Translational Modification
IRG1 has an amino acid length of 481, molecular weight is 52628 Da.
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Subunit
Homodimer
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SwissProt ID
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Synonyms
ACOD1, Aconitate decarboxylase 1, Aconitate decarboxylase, CAD, Cis aconitate decarboxylase, Immune responsive gene 1 protein homolog, Immune-responsive gene 1 protein homolog, Immunoresponsive 1 homolog (mouse), Irg1, IRG1_HUMAN
Documentation
References
[1]. Li Y, et al. Immune responsive gene 1 (IRG1) promotes endotoxin tolerance by increasing A20 expression in macrophages through reactive oxygen species. J Biol Chem. 2013 Jun 7;288(23):16225-16234. [Content Brief]
[2]. Michelucci A, et al. Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7820-5. [Content Brief]
[3]. Chen F, et al. Crystal structure of cis-aconitate decarboxylase reveals the impact of naturally occurring human mutations on itaconate synthesis. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20644-20654. [Content Brief]
[4]. Zhang Z, et al. Itaconate is a lysosomal inducer that promotes antibacterial innate immunity. Mol Cell. 2022 Aug 4;82(15):2844-2857.e10. [Content Brief]
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