Gemin 2 Antibody (YA2906)
(Synonyms: gemin2; SIP 1; SIP1; SIP1 delta; SMN interacting protein 1; Survival interacting protein 1)Gemin 2 Antibody (YA2906) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gemin 2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50 |
Product Details
Gemin 2 Antibody (YA2906) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gemin 2.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 32 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32 kDa
Entrez Gene: 8487 Human ; 66603 Mouse ; 84404 Rat
SwissProt: O14893 Human ; Q9CQQ4 Mouse ; Q9QZP1 Rat
OMIM: 602595 Human
A synthesized peptide derived from human Gemin 2 aa11-71.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Gemin 2 is a The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound[1][2][3][4][5].
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Subcellular Localization
Nucleus, gem; Cytoplasm
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Isoforms & Post-Translational Modification
Gemin 2 has 5 isomers: O14893-1: 31585 Da (predicted); O14893-2: 29931 Da (predicted); O14893-3: 28155 Da (predicted); O14893-4: 4826 Da (predicted); O14893-5: 30358 Da (predicted).
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Subunit
Monomer. Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts with GEMIN5; the interaction is direct. Interacts (via C-terminus) with SMN1; the interaction is direct. Interacts with SNRPD1; the interaction is direct. Interacts with SNRPD2; the interaction is direct. Interacts (via N-terminus) with SNRPF; the interaction is direct. Interacts (via N-terminus) with SNRPE; the interaction is direct. Interacts (via N-terminus) with SNRPG; the interaction is direct.
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SwissProt ID
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Synonyms
gemin2; SIP 1; SIP1; SIP1 delta; SMN interacting protein 1; Survival interacting protein 1
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Research Field
Neuroscience
Documentation
References
[1]. Chari A, et al. An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell. 2008 Oct 31;135(3):497-509. [Content Brief]
[2]. Liu Q, et al. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell. 1997 Sep 19;90(6):1013-21. [Content Brief]
[3]. Yi H, et al. Negative cooperativity between Gemin2 and RNA provides insights into RNA selection and the SMN complex's release in snRNP assembly. Nucleic Acids Res. 2020 Jan 24;48(2):895-911. [Content Brief]
[4]. Golembe TJ, et al. Specific sequence features, recognized by the SMN complex, identify snRNAs and determine their fate as snRNPs. Mol Cell Biol. 2005 Dec;25(24):10989-1004. [Content Brief]
[5]. Zhang R, et al. Structure of a key intermediate of the SMN complex reveals Gemin2's crucial function in snRNP assembly. Cell. 2011 Aug 5;146(3):384-95. [Content Brief]
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