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TFAP2A Antibody (YA3659)

(Synonyms: AP-2; BOFS; AP2TF; TFAP2; AP-2alpha)
Customer Review

Based on 1 Customer Validation

TFAP2A Antibody (YA3659) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TFAP2A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • TFAP2A Antibody (YA3659) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

TFAP2A Antibody (YA3659) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TFAP2A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Immunogen

Purified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718803

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TFAP2A Antibody (YA3659)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), SiHa (lane 4(20μg) and MCF-7 (lane 5(20μg) using TFAP2A Antibody (HY-P83962) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
    Flow cytometric analysis of 1X106 HeLa cells labeling TFAP2A Antibody (HY-P83962, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    TFAP2A is a Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Human (P05549) has 4 isomers: P05549-1: 48,062 Da (predicted); P05549-2: 40,557 Da (predicted); P05549-5: 47,183 Da (predicted); P05549-6: 47,440 Da (predicted).
    Sumoylated on Lys-10; which inhibits transcriptional activity.

  • Subunit

    Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Interacts with KCTD15; this interaction inhibits TFAP2A transcriptional activation

  • SwissProt ID

    P05549

  • Gene ID
    7020 [NCBI]
  • Synonyms

    AP-2; BOFS; AP2TF; TFAP2; AP-2alpha

TFAP2A Antibody (YA3659) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), SiHa (lane 4(20μg) and MCF-7 (lane 5(20μg) using TFAP2A Antibody (HY-P83962) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling TFAP2A Antibody (HY-P83962, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TFAP2A Antibody (YA3659) Product Image
    1/16
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • TFAP2A Antibody (YA3659) Product Image
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), SiHa (lane 4(20μg) and MCF-7 (lane 5(20μg) using TFAP2A Antibody (HY-P83962) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling TFAP2A Antibody (HY-P83962, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • Experimental Validation Results for TFAP2A Antibody (YA3659)
  • TFAP2A Antibody (YA3659) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), SiHa (lane 4(20μg) and MCF-7 (lane 5(20μg) using TFAP2A Antibody (HY-P83962) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TFAP2A Antibody (YA3659) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling TFAP2A Antibody (HY-P83962, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TFAP2A Antibody (YA3659) Product Image WB
  • TFAP2A Antibody (YA3659) Product Image FC