CD5 Antibody (YA5588)
(Synonyms: CD 5; CD5; CD5 antigen; p56 62; CD5 antigen; CD5 molecule; CD5_HUMAN; LEU1; LyA; Lymphocyte antigen T1/Leu-1; OTTHUMP00000236973; T-cell surface glycoprotein CD5; T1)CD5 Antibody (YA5588) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD5.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
IHC-P, WB, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
CD5 Antibody (YA5588) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD5.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
Synthesized peptide derived from human CD5 AA range: 200-300
Protein G
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD5 is a Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production[1][2][3][4].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Subunit
Interacts with CD72/LYB-2 (PubMed:1711157). Interacts with PTPN6/SHP-1 (By similarity). Interacts with CBL (PubMed:23376399). Interacts with CD5L (By similarity). Interacts with CD3Z/CD247 (PubMed:1384049, PubMed:1385158)
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SwissProt ID
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Synonyms
CD 5; CD5; CD5 antigen; p56 62; CD5 antigen; CD5 molecule; CD5_HUMAN; LEU1; LyA; Lymphocyte antigen T1/Leu-1; OTTHUMP00000236973; T-cell surface glycoprotein CD5; T1
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