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Ovalbumin Antibody (YA3433)

(Synonyms: OVA,Ovalbumin )
Customer Review

Based on 1 Customer Validation

Ovalbumin Antibody (YA3433) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ovalbumin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Chicken

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ovalbumin Antibody (YA3433)
  • Ovalbumin Antibody (YA3433) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

Ovalbumin Antibody (YA3433) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ovalbumin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Chicken
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database

Entrez Gene: 396058 Chicken

SwissProt: P01012 Chicken

Immunogen

Ovalbumin

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3676583

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ovalbumin Antibody (YA3433)
    Western blot analysis was performed on protein extracts from Ovalbumins (lane 2, 0.5 μg), Ovalbumins (lane 3, 1 μg), and Ovalbumins (lane 4, 2 μg) using Ovalbumin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Ovalbumin is a Non-inhibitory serpin. Storage protein of egg white

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:The main protein in egg white. Expressed in the ampulla of the fallopian tube (protein level) (PubMed:25436390)

    Induction:Down-regulated by dietary stress. Decreased expression at day 14 in the magnum of the oviduct in the corticosterone-fed laying hens

  • Subunit

    Homodimer

  • SwissProt ID

    P01012

  • Gene ID
    396058 [NCBI]
  • Synonyms

    OVA,Ovalbumin

  • Research Field

    Cell Biology

Ovalbumin Antibody (YA3433) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Ovalbumins (lane 2, 0.5 μg), Ovalbumins (lane 3, 1 μg), and Ovalbumins (lane 4, 2 μg) using Ovalbumin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Ovalbumin Antibody (YA3433) Product Image
    1/16
  • Experimental Validation Results for Ovalbumin Antibody (YA3433)
  • Ovalbumin Antibody (YA3433) Product Image
  • Experimental Validation Results for Ovalbumin Antibody (YA3433)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Ovalbumins (lane 2, 0.5 μg), Ovalbumins (lane 3, 1 μg), and Ovalbumins (lane 4, 2 μg) using Ovalbumin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Ovalbumin Antibody (YA3433)
  • Ovalbumin Antibody (YA3433) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts from Ovalbumins (lane 2, 0.5 μg), Ovalbumins (lane 3, 1 μg), and Ovalbumins (lane 4, 2 μg) using Ovalbumin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Ovalbumin Antibody (YA3433) Product Image WB