Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407)

Cat. No.: HY-P80407: Data Sheet COA
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Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamate Receptor 1 (AMPA Subtype).

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutamate Receptor 1 (AMPA Subtype).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 90 kDa;

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat

SwissProt ID

P42261

Gene ID

2890 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Glutamate receptor 1.AA range:857-906.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Glutamate Receptor 1 (AMPA Subtype) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Glutamate Receptor 1 (AMPA Subtype) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:1311100, PubMed:20805473, PubMed:21172611, PubMed:28628100, PubMed:35675825). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611). Resensitization is blocked by CNIH2 tH2O2gh interaction with CACNG8 in the CACNG8-containing AMPA receptors complex (PubMed:21172611). Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+) (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Postsynaptic cell membrane; Multi-pass membrane protein; Postsynaptic density membrane; Multi-pass membrane protein; Cell projection, dendrite; Cell projection, dendritic spine; Early endosome membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein; Presynapse; Synapse

Expression：
Tissue_specificity:Widely expressed in the brain

Subunit：Homotetramer or heterotetramer of pore-forming glutamate receptor subunits; heteromeric assembly can be the result of both receptor subtype and flip or flop form and according the composition, one partner can be dominant with respect to the fast desensitizing current component, whereas the other can determine the steady-state component (By similarity). Tetramers may be formed by the dimerization of dimers (PubMed:23739980). Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity). Interacts with HIP1 and RASGRF2. Interacts with SYNDIG1 and GRIA2 (By similarity). Interacts with DLG1 (via C-terminus). Interacts with LRFN1. Interacts with PRKG2. Interacts with CNIH2 and CACNG2 (PubMed:20805473). Interacts with CACNG5; this interaction modulates the gating. Interacts (via C-terminus) with PDLIM4 (via LIM domain); this interaction as well as the interaction of PDLIM4 with alpha-actinin is required for their colocalization in early endosomes (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain) (By similarity). Interacts with CACNG3; associates GRIA1 with the adapter protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons (By similarity). Interacts with CACNG2; this interaction mediates traffick to the plasma membrane and modulation of desensitization. Interacts with CNIH2 and CNIH3; this interaction promotes expression at the plasma membrane and extensively modulates their gating properties by slowing deactivation and desensitization kinetics (By similarity). Found in a complex with GRIA2, GRIA3, GRIA4, DLG4, CACNG8 and CNIH2 (By similarity)

RRID

AB_3102491

Database

Entrez Gene: 2890 Human ; 14799 Mouse ; 50592 Rat

SwissProt: P42261 Human ; P23818 Mouse ; P19490 Rat

OMIM: 138248 Human

Research Field

Neuroscience

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407)

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Glutamate Receptor 1 (AMPA Subtype) Antibody (YA407) Related Classifications

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