ARHGEF1 Antibody
(Synonyms: Lbcl2, Lsc, Arhgef1, Rho guanine nucleotide exchange factor 1, Lbc's second cousin, Lymphoid blast crisis-like 2)ARHGEF1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARHGEF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
ARHGEF1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARHGEF1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 125; 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 102 kDa
Synthetic peptide corresponding to the center region of human ARHGEF1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ARHGEF1 is a Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. In lymphoid follicles, may trigger activation of GNA13 as part of S1PR2-dependent signaling pathway that leads to inhibition of germinal center (GC) B cell growth and migration outside the GC niche[1][2][3][4][5].
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Subcellular Localization
Cytoplasm; Membrane
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Expression
Tissue_Specificity: Ubiquitously expressed. -
Isoforms & Post-Translational Modification
ARHGEF1 has 4 isoforms, Q92888-1: amino acid length is 912, molecular weight is 102435 Da (predicted); Q92888-2: amino acid length is 879, molecular weight is 98768 Da (predicted); Q92888-3: amino acid length is 927, molecular weight is 104066 Da (predicted); Q92888-4: amino acid length is 948, molecular weight is 105854 Da (predicted).
Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. -
Subunit
Interacts with RHOA, GNA12 and GNA13.
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SwissProt ID
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Synonyms
Lbcl2, Lsc, Arhgef1, Rho guanine nucleotide exchange factor 1, Lbc's second cousin, Lymphoid blast crisis-like 2
Documentation
[1]. Kozasa T, et al. p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Science. 1998 Jun 26;280(5372):2109-11. [Content Brief]
[2]. Hart MJ, et al. Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13. Science. 1998 Jun 26;280(5372):2112-4. [Content Brief]
[3]. Bouafia A, et al. Loss of ARHGEF1 causes a human primary antibody deficiency. J Clin Invest. 2019 Mar 1;129(3):1047-1060. [Content Brief]
[4]. Hart MJ, et al. Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. J Biol Chem. 1996 Oct 11;271(41):25452-8. [Content Brief]
[5]. Guilluy C, et al. The Rho exchange factor Arhgef1 mediates the effects of angiotensin II on vascular tone and blood pressure. Nat Med. 2010 Feb;16(2):183-90. [Content Brief]
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