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PDIA6 Antibody (YA237)

(Synonyms: ERP5, P5, TXNDC7, PDIA6, Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5)
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Based on 1 Customer Validation

PDIA6 Antibody (YA237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDIA6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IHC-F, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PDIA6 Antibody (YA237)
  • PDIA6 Antibody (YA237) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-2000 1:50-200 1:100-500 1:100-500 1:100-500

Product Details

Description

PDIA6 Antibody (YA237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDIA6.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 48kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PDIA6. The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDIA6 Antibody (YA237)
    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HeLa (lane 3), HepG2 (lane 4), Raji (lane 5) and 293T (lane 6) using PDIA6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    PDIA6 may function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin[1][2][3].

  • Subcellular Localization

    Endoplasmic reticulum lumen; Cell membrane; Melanosome

  • Expression


    Tissue_specificity:Expressed in platelets (at protein level)

  • Subunit

    Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX (PubMed:12475965). Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells (PubMed:17495932). Interacts with ITGB3 following platelet stimulation (PubMed:15466936). Interacts with ERN1; the interaction is direct (PubMed:24508390). Interacts with EIF2AK3 (PubMed:24508390)

  • SwissProt ID

    Q15084

  • Gene ID
    10130 [NCBI]
  • Synonyms

    ERP5, P5, TXNDC7, PDIA6, Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5

References

[1]. Kikuchi M, et al. Functional analysis of human P5, a protein disulfide isomerase homologue. J Biochem. 2002 Sep;132(3):451-5. [Content Brief]

[2]. Eletto D, et al. Protein disulfide isomerase A6 controls the decay of IRE1α signaling via disulfide-dependent association. Mol Cell. 2014 Feb 20;53(4):562-576. [Content Brief]

[3]. Jordan PA, et al. A role for the thiol isomerase protein ERP5 in platelet function. Blood. 2005 Feb 15;105(4):1500-7. [Content Brief]

PDIA6 Antibody (YA237) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HeLa (lane 3), HepG2 (lane 4), Raji (lane 5) and 293T (lane 6) using PDIA6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • PDIA6 Antibody (YA237) Product Image
    1/16
  • Experimental Validation Results for PDIA6 Antibody (YA237)
  • PDIA6 Antibody (YA237) Product Image
  • Experimental Validation Results for PDIA6 Antibody (YA237)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HeLa (lane 3), HepG2 (lane 4), Raji (lane 5) and 293T (lane 6) using PDIA6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for PDIA6 Antibody (YA237)
  • PDIA6 Antibody (YA237) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), HeLa (lane 3), HepG2 (lane 4), Raji (lane 5) and 293T (lane 6) using PDIA6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • PDIA6 Antibody (YA237) Product Image WB