Copy Link Print Information

ROMO1 Antibody (YA8354)

(Synonyms: bA353C18.2; C20orf52; MTGM; MTGMP)
Customer Review

Based on 1 Customer Validation

ROMO1 Antibody (YA8354) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ROMO1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat, Monkey, Dog

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • ROMO1 Antibody (YA8354) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:150-500 1:100-250

Product Details

Description

ROMO1 Antibody (YA8354) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ROMO1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Dog
  • Calculated Molecular Weight Predicted band size: 8 kDa
Immunogen

Full length human recombinant protein of human ROMO1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ROMO1 Antibody (HY-P88670). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    Immunocytochemistry analysis of Hela cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    Immunocytochemistry analysis of PC-3 cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ROMO1 induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation|Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage

  • Subcellular Localization

    Mitochondrion inner membrane

  • Expression


    Tissue_Specificity: Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line. Highly expressed in brain tumors
    Induction: By the anticancer drug fluorouracil (5FU)

  • Isoforms & Post-Translational Modification

    P60602 has two isomers: P60602-1: 8183 Da (predicted); P60602-2: 5900 Da (predicted).

  • SwissProt ID

    P60602

  • Synonyms

    bA353C18.2; C20orf52; MTGM; MTGMP

ROMO1 Antibody (YA8354) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ROMO1 Antibody (HY-P88670). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROMO1 Antibody (YA8354) Product Image
    1/16
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • ROMO1 Antibody (YA8354) Product Image
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ROMO1 Antibody (HY-P88670). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • Experimental Validation Results for ROMO1 Antibody (YA8354)
  • ROMO1 Antibody (YA8354) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2(20μg), Hela (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using ROMO1 Antibody (HY-P88670). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • ROMO1 Antibody (YA8354) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROMO1 Antibody (YA8354) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling ROMO1 with ROMO1 Antibody (HY-P88670) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ROMO1 Antibody (HY-P88670) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ROMO1 Antibody (YA8354) Product Image WB
  • ROMO1 Antibody (YA8354) Product Image ICC
  • ROMO1 Antibody (YA8354) Product Image ICC