Copy Link Print Information

ALP Antibody (YA6876)

(Synonyms: Actinin associated LIM protein antibody; PDZ and LIM domain 3 antibody; PDLIM 3 antibody)
Customer Review

Based on 1 Customer Validation

ALP Antibody (YA6876) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ALP Antibody (YA6876)
  • Experimental Validation Results for ALP Antibody (YA6876)
  • ALP Antibody (YA6876) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1-2μg/sample

Product Details

Description

ALP Antibody (YA6876) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：39 kDa
Immunogen

Recombinant protein within human ALP aa 1-364.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719287

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ALP Antibody (YA6876)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HT1080 (lane 2(20μg)) 、C2C12 (lane 3(20μg)) and Rat heart (lane 4(20μg)) using ALP Antibody (HY-P87188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ALP Antibody (YA6876)
    Western blot analysis of extracts from Saos-2 (lane2, 30μg) using ALP Antibody (HY-P87188). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ALP may play a role in the organization of actin filament arrays within muscle cells

  • Subcellular Localization

    Cytoplasm, myofibril, sarcomere, Z line

  • Expression


    Tissue_Specificity: Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific

  • Isoforms & Post-Translational Modification

    Q53GG5 has three isomers: Q53GG5-1: 39232 Da (predicted); Q53GG5-2: 34280 Da (predicted); Q53GG5-3: 20214 Da (predicted).

  • Subunit

    Interacts with ACTN2 (By similarity)

  • SwissProt ID

    Q53GG5

  • Synonyms

    Actinin associated LIM protein antibody; PDZ and LIM domain 3 antibody; PDLIM 3 antibody

ALP Antibody (YA6876) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HT1080 (lane 2(20μg)) 、C2C12 (lane 3(20μg)) and Rat heart (lane 4(20μg)) using ALP Antibody (HY-P87188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane2, 30μg) using ALP Antibody (HY-P87188). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ALP Antibody (YA6876) Product Image
    1/16
  • Experimental Validation Results for ALP Antibody (YA6876)
  • Experimental Validation Results for ALP Antibody (YA6876)
  • ALP Antibody (YA6876) Product Image
  • Experimental Validation Results for ALP Antibody (YA6876)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HT1080 (lane 2(20μg)) 、C2C12 (lane 3(20μg)) and Rat heart (lane 4(20μg)) using ALP Antibody (HY-P87188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ALP Antibody (YA6876)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Saos-2 (lane2, 30μg) using ALP Antibody (HY-P87188). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ALP Antibody (YA6876)
  • Experimental Validation Results for ALP Antibody (YA6876)
  • ALP Antibody (YA6876) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HT1080 (lane 2(20μg)) 、C2C12 (lane 3(20μg)) and Rat heart (lane 4(20μg)) using ALP Antibody (HY-P87188) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ALP Antibody (YA6876) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Saos-2 (lane2, 30μg) using ALP Antibody (HY-P87188). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ALP Antibody (YA6876) Product Image WB
  • ALP Antibody (YA6876) Product Image WB