Copy Link Print Information

HuC/HuD Antibody (YA3060)(PBS only)

(Synonyms: Huc; mHuC; PLE21; 2600009P04Rik)

HuC/HuD Antibody (YA3060) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HuC/HuD.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HuC/HuD Antibody (YA3060)(PBS only)
  • HuC/HuD Antibody (YA3060)(PBS only) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

HuC/HuD Antibody (YA3060) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HuC/HuD.

  • Host Rabbit
  • Species Reactivity
    Rat
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Immunogen

Recombinant protein of mouse HuC

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HuC/HuD Antibody (YA3060)(PBS only)
    Western blot analysis was performed on extracts from Rat brain (lane 1, 15 μg), Rat cerebellum (lane 2, 15 μg), and Mouse brain (lane 3, 15 μg) using HuC/HuD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    HuC/HuD is a Q60900: RNA-binding protein that binds to AU-rich element (ARE) sequences of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance. Plays a role in the stabilization of GAP43 mRNA and in spatial learning
    P26378: RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs. Plays a role in the regulation of mRNA stability, alternative splicing and translation. Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA. By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay. Also binds to the polyadenylated (poly (A) ) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding. Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms.
    [1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    P26378: Cytoplasm; Perikaryon; Cell projection, dendrite; Cell projection, axon; Cell projection, growth cone

  • Expression


    Tissue_specificity: Q60900: Brain specific (PubMed:9016658). Expressed in the hippocampus with expression in CA1, CA3 and dentate gyrus (PubMed:11573004)
    P26378: Expressed in pancreatic beta cells (at protein level) (PubMed:22387028). Expressed in the brain (PubMed:14702039, PubMed:1655278)
    Induction: Q60900: Up-regulated after spatial learning in radial arm maze experiments and in Morris water maze experiments

  • Isoforms & Post-Translational Modification

    Q60900 has 2 isomers: Q60900-1: 39533 Da (predicted); Q60900-2: 38851 Da (predicted).

  • Subunit

    Q60900: Interacts with MAP1B light chain LC1
    P26378: Interacts with SMN (via Tudor domain) in an RNA-independent manner; the interaction is required for localization of ELAVL4 to RNA granules (PubMed:21088113, PubMed:21389246, PubMed:29061699) .

  • SwissProt ID

    Q60900 / P26378

  • Gene ID
    15571 [NCBI]
  • Synonyms

    Huc; mHuC; PLE21; 2600009P04Rik

  • Research Field

    Neuroscience

HuC/HuD Antibody (YA3060)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat brain (lane 1, 15 μg), Rat cerebellum (lane 2, 15 μg), and Mouse brain (lane 3, 15 μg) using HuC/HuD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • HuC/HuD Antibody (YA3060)(PBS only) Product Image
    1/16
  • Experimental Validation Results for HuC/HuD Antibody (YA3060)(PBS only)
  • HuC/HuD Antibody (YA3060)(PBS only) Product Image
  • Experimental Validation Results for HuC/HuD Antibody (YA3060)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat brain (lane 1, 15 μg), Rat cerebellum (lane 2, 15 μg), and Mouse brain (lane 3, 15 μg) using HuC/HuD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for HuC/HuD Antibody (YA3060)(PBS only)
  • HuC/HuD Antibody (YA3060)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Rat brain (lane 1, 15 μg), Rat cerebellum (lane 2, 15 μg), and Mouse brain (lane 3, 15 μg) using HuC/HuD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • HuC/HuD Antibody (YA3060)(PBS only) Product Image WB