Integrin alpha 4/CD49D Antibody (YA3782)
(Synonyms: ITGA4; IA4)Integrin alpha 4/CD49D Antibody (YA3782) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin alpha 4/CD49D.
-
Host:
Mouse
-
Isotype:
IgG2a
-
Application:
WB, FC, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS with 0.05% sodium azide
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
Integrin alpha 4/CD49D Antibody (YA3782) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Integrin alpha 4/CD49D.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 115 kDa
Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS with 0.05% sodium azide
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Integrin alpha 4/CD49D is an Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. Integrin ITGA4:ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction[1][2][3][4].
-
Subcellular Localization
Membrane; Single-pass type I membrane protein
-
Expression
Tissue_specificity:Expression (protein level) in vascular smooth muscle cells. -
Isoforms & Post-Translational Modification
P13612 has 2 isomers: P13612-1: 114900 Da (predicted); P13612-2: 21536 Da (predicted).
Phosphorylation on Ser-1027 inhibits PXN binding -
Subunit
Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with JAML; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization. ITGA4:ITGB1 is found in a ternary complex with CX3CR1 and CX3CL1 (PubMed:23125415). Interacts with MDK (PubMed:15466886). ITGA4:ITGB1 interacts with MDK; this interaction mediates MDK-induced osteoblast cells migration through PXN phosphorylation (PubMed:15466886). Integrin ITGA4:ITGB1 interacts with SVEP1 (via Sushi domain 21); thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction (PubMed:35802072). ITGA4:ITGB1 interacts with SELP (PubMed:37184585). ITGA4:ITGB1 interacts with BCAM (PubMed:17158232)
-
SwissProt ID
-
Synonyms
ITGA4; IA4
Documentation
Powered by Bioz
