RALBP1 Antibody (YA3053)

Cat. No.: HY-P83308: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

RALBP1 Antibody (YA3053) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RALBP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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RALBP1 Antibody (YA3053) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RALBP1 Antibody (YA3053) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RALBP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 76 kDa;

Observed band size: 95 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15311

Gene ID

10928 [NCBI]

Immunogen

A synthesized peptide derived from human RALBP1 aa200-265/655.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed:7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed:7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed:10910768, PubMed:12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed:12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed:21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed:21822277); Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasm, cytosol; Cytoplasm, cytoskeleton, spindle pole; Nucleus; Mitochondrion

Expression：
Tissue_specificity:Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes

Subunit：Interacts with the GTP-bound form of RALA (via effector domain); during mitosis, recruits RALBP1 to the mitochondrion where it promotes DNM1L phosphorylation and mitochondrial fission (PubMed:21822277, PubMed:7673236). Interacts with DNM1L; mediates its mitotic kinase cyclin B-CDK1-mediated phosphorylation during mitosis to promote mitochondrial fission (PubMed:21822277). Interacts with the mitotic kinase cyclin B-CDK1 during mitosis (PubMed:12775724, PubMed:21822277). Interacts with the GTP-bound form of RALB (via effector domain) (PubMed:20696399, PubMed:7673236). Interacts with REPS1; the interaction is direct and does not affect RALA-binding nor GTPase activator activity of RALBP1 (By similarity). Interacts with REPS2; the interaction is direct and does not affect RALA-binding nor GTPase activator activity of RALBP1 (PubMed:9422736). Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis (PubMed:12775724). Interacts with AP2M1; as part of the AP2 complex (PubMed:10910768). Interacts with CDC42 (Probable). Interacts with RAC1 (PubMed:7673236)

Database

Entrez Gene: 10928 Human ; 19765 Mouse ; 84014 Rat

SwissProt: Q15311 Human ; Q62172 Mouse ; Q62796 Rat

OMIM: 605801 Human

Research Field

Cell Biology

Synonyms

RALBP1; RIP1; RLIP1; RLIP76

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

RALBP1 Antibody (YA3053) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RALBP1 Antibody (YA3053)

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Background

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RALBP1 Antibody (YA3053) Related Classifications

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