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Chromogranin A Antibody (YA5572)

(Synonyms: beta Granin antibody; betagranin; N-terminal fragment of chromogranin A; antibody; catestatin antibody; CgA antibody; CHG A antibody; Chga antibody; chromofungin antibody; Chromogranin A antibody; Chromogranin A parathyroid secretory protein 1 antibody; Chromogranin A precursor antibody; ChromograninA antibody; CMGA_HUMAN antibody; ER-37 antibody; Pancreastatin antibody; Parastatin antibody; Parathyroid secretory protein 1 antibody; Pituitary secretory protein I antibody; Secretory protein I antibody; SP I antibody; SP-I antibody; SP1 antibody; SPI antibody; vasostatin 2 antibody; Vasostatin antibody; Vasostatin I antibody; Vasostatin II antibody; vasostatin-2 antibody; )
Customer Review

Based on 1 Customer Validation

Chromogranin A Antibody (YA5572) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Chromogranin A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Chromogranin A Antibody (YA5572) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

Chromogranin A Antibody (YA5572) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Chromogranin A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Immunogen

Synthesized peptide derived from human Chromogranin A AA range: 19-94

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Chromogranin A is a Strongly inhibits glucose induced insulin release from the pancreas; Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure; Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation[1][2][3][4][5].

  • Subcellular Localization

    Secreted; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle; Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in cerebrospinal fluid (PubMed: 25326458) . Protein levels were detected in urine (PubMed: 37453717) ; it was also found in brain tissue.

  • Subunit

    Self-interacts; self-assembly is promoted in vitro by chondroitin sulfate attachment which occurs at mildly acidic pH conditions (PubMed:25326458).

  • SwissProt ID

    P10645

  • Gene ID
    1113 [NCBI]
  • Synonyms

    beta Granin antibody; betagranin; N-terminal fragment of chromogranin A; antibody; catestatin antibody; CgA antibody; CHG A antibody; Chga antibody; chromofungin antibody; Chromogranin A antibody; Chromogranin A parathyroid secretory protein 1 antibody; Chromogranin A precursor antibody; ChromograninA antibody; CMGA_HUMAN antibody; ER-37 antibody; Pancreastatin antibody; Parastatin antibody; Parathyroid secretory protein 1 antibody; Pituitary secretory protein I antibody; Secretory protein I antibody; SP I antibody; SP-I antibody; SP1 antibody; SPI antibody; vasostatin 2 antibody; Vasostatin antibody; Vasostatin I antibody; Vasostatin II antibody; vasostatin-2 antibody;

Chromogranin A Antibody (YA5572) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Chromogranin A Antibody (YA5572) Product Image
    1/16
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Chromogranin A Antibody (YA5572) Product Image
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Experimental Validation Results for Chromogranin A Antibody (YA5572)
  • Chromogranin A Antibody (YA5572) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Chromogranin A Antibody (YA5572) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Chromogranin A (PTR1330) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Chromogranin A Antibody (YA5572) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Chromogranin A Antibody (YA5572) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Chromogranin A Antibody (HY-P85880) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Chromogranin A Antibody (HY-P85880) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Chromogranin A Antibody (YA5572) Product Image IHC-P
  • Chromogranin A Antibody (YA5572) Product Image IHC-P
  • Chromogranin A Antibody (YA5572) Product Image ICC
  • Chromogranin A Antibody (YA5572) Product Image ICC