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Vimentin Antibody (YA6418)

(Synonyms: FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.)
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Based on 1 Customer Validation

Vimentin Antibody (YA6418) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Vimentin Antibody (YA6418)
  • Vimentin Antibody (YA6418) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-10000 1:200-1000 1:200-1000 1:200-1:1000 1:100-500 1ug:Test

Product Details

Description

Vimentin Antibody (YA6418) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 53 kDa
Immunogen

Recombinant human Vimentin Protein

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vimentin Antibody (YA6418)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), SH-SY5Y (lane 3), A549 (lane 4), HT-1080 (lane 5), THP-1 (lane 6) and Raji (lane 7, Negative) using Vimentin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Vimentin is a Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner|Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2

  • Subcellular Localization

    Cytoplasm,Cytoplasm, cytoskeleton,Nucleus matrix,Cell membrane

  • Expression


    Tissue_Specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines
    Induction: Up-regulated by muramyl-dipeptide and lipopolysaccharide

  • Isoforms & Post-Translational Modification

    P08670: 466 amino acids, molecular weight 53652 Da.
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake丨Palmitoylated by ZDHHC16, promoting formation of the homopentamer丨In elongated spermatids, proteolytically cleaved by SPPL2C which modulates intracellular Ca(2+) homeostasis

  • Subunit

    Homomer assembled from elementary dimers (PubMed:20176112)

  • SwissProt ID

    P08670

  • Gene ID
    7431 [NCBI]
  • Synonyms

    FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Vimentin Antibody (YA6418) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), SH-SY5Y (lane 3), A549 (lane 4), HT-1080 (lane 5), THP-1 (lane 6) and Raji (lane 7, Negative) using Vimentin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Vimentin Antibody (YA6418) Product Image
    1/16
  • Experimental Validation Results for Vimentin Antibody (YA6418)
  • Vimentin Antibody (YA6418) Product Image
  • Experimental Validation Results for Vimentin Antibody (YA6418)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), SH-SY5Y (lane 3), A549 (lane 4), HT-1080 (lane 5), THP-1 (lane 6) and Raji (lane 7, Negative) using Vimentin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Vimentin Antibody (YA6418)
  • Vimentin Antibody (YA6418) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), SH-SY5Y (lane 3), A549 (lane 4), HT-1080 (lane 5), THP-1 (lane 6) and Raji (lane 7, Negative) using Vimentin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Vimentin Antibody (YA6418) Product Image WB