Vimentin Antibody (YA6418)

Cat. No.: HY-P86726: Data Sheet COA
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Vimentin Antibody (YA6418) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Vimentin Antibody (YA6418) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Vimentin.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 53 kDa

Species Reactivity

Human

SwissProt ID

P08670

Gene ID

7431 [NCBI]

Immunogen

Recombinant human Vimentin Protein

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200-1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
FC FC: Flow Cytometry	1ug:Test

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), SH-SY5Y (lane 3), A549 (lane 4), HT-1080 (lane 5), THP-1 (lane 6) and Raji (lane 7, Negative) using Vimentin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity)|Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2

Subcellular Localization：Cytoplasm,Cytoplasm, cytoskeleton,Nucleus matrix,Cell membrane

Expression：
Tissue_Specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines
Induction: Up-regulated by muramyl-dipeptide and lipopolysaccharide

Isoforms & Post-Translational Modification：P08670: 466 amino acids, molecular weight 53652 Da.
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake丨Palmitoylated by ZDHHC16, promoting formation of the homopentamer丨In elongated spermatids, proteolytically cleaved by SPPL2C which modulates intracellular Ca(2+) homeostasis

Subunit：Homomer assembled from elementary dimers (PubMed:20176112)

Synonyms

FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)