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RNase L Antibody (YA2314)

(Synonyms: PRCA1; Ribonuclease4; RibonucleaseL; RNase L; Rnasel; RNS4)
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Based on 1 Customer Validation

RNase L Antibody (YA2314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNase L.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • RNase L Antibody (YA2314) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

RNase L Antibody (YA2314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RNase L.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Species Reactivity Database

Entrez Gene: 6041 Human

SwissProt: Q05823 Human

Immunogen

A synthesized peptide derived from human RNase L aa550-741/741.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104670

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RNase L Antibody (YA2314)
    Western blot analysis of extracts from THP-1(lane 2(20ug) and THP-1(lane 3(40ug) using RNase L Antibody (HY-P82569) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RNase L Antibody (YA2314)
    Western blot analysis of extracts from HEK293T (lane2, 20μg), THP-1 (lane3, 20μg), K562 (lane4, 20μg) and HCT116 (lane5, 20μg) using RNase L Antibody (HY-P82569). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RNase L Antibody (YA2314)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HL-60 (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using RNase L Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    RNASEL encodes RNase L, an interferon-inducible endoribonuclease that functions as a central effector of the 2′-5′-oligoadenylate synthetase (OAS) -RNase L pathway, a conserved arm of innate antiviral immunity[1][2]. Upon recognition of viral double-stranded RNA, OAS enzymes synthesize 2′-5′-linked oligoadenylates (2-5A), which bind and activate RNase L, leading to the cleavage of cellular and viral RNA and suppression of viral replication[1][3][4]. Mechanistically, RNase L-mediated RNA fragmentation not only restricts pathogen replication but also promotes downstream stress and immune signaling, including activation of ZAKα-dependent ribotoxic stress pathways and antiviral transcriptional programs[2]. Beyond classical antiviral defense, the OAS-RNase L axis regulates inflammatory responses, and genetic defects in this pathway have been linked to severe inflammatory manifestations associated with SARS-CoV-2 infection, highlighting its importance in immune homeostasis[5]. Disease-relevant studies further demonstrate that RNase L participates in tissue-specific biological processes, including the regulation of wound-induced hair follicle neogenesis and epithelial regeneration through modulation of innate immune signaling pathways[6]. Compared with related OAS family members that function primarily as upstream sensors and 2-5A synthetases, RNase L serves as the terminal catalytic effector that directly executes RNA degradation after pathway activation[1][3]. For experimental applications, pharmacological inhibition of RNase L has been achieved using small-molecule inhibitors that block RNase L activity and prevent excessive RNA cleavage, providing useful tools for mechanistic studies of innate immunity, inflammation, and RNA biology[7].

  • Subcellular Localization

    Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:Highly expressed in spleen and thymus followed by prostate, testis, uterus, small intestine, colon and peripheral blood leukocytes

    Induction:By interferons. Virus replication in higher vertebrates is restrained by IFNs that cause cells to transcribe genes encoding antiviral proteins, such as 2'-5' oligoadenylate synthetases (OASs) . oligoadenylate synthetase is stimulated by dsRNA to produce 5'-phosphorylated, 2'-5'-linked oligoadenylates (2-5A) , whose function is to activate RNASEL

  • Isoforms & Post-Translational Modification

    Q05823 has 2 isomers: Q05823-1: 83533 Da (predicted); Q05823-2: 73416 Da (predicted).

  • Subunit

    Monomer (inactive form) or homodimer. Interacts with ABCE1; this interaction inhibits the RNASEL

  • SwissProt ID

    Q05823

  • Gene ID
    6041 [NCBI]
  • Synonyms

    PRCA1; Ribonuclease4; RibonucleaseL; RNase L; Rnasel; RNS4

  • Research Field

    Immunology

References

[1]. Karasik A, et al. The Unusual Role of Ribonuclease L in Innate Immunity. Wiley Interdiscip Rev RNA. 2024 Nov-Dec;15(6):e1878. [Content Brief]

[2]. Karasik A, et al. Endonucleolytic RNA cleavage drives changes in gene expression during the innate immune response. Cell Rep. 2024 Jun 25;43(6):114287. [Content Brief]

[3]. Soveg FW, et al. Endomembrane targeting of human OAS1 p46 augments antiviral activity. Elife. 2021 Aug 3;10:e71047. [Content Brief]

[4]. Ezelle HJ, et al. The Roles of RNase-L in Antimicrobial Immunity and the Cytoskeleton-Associated Innate Response. Int J Mol Sci. 2016 Jan 8;17(1):74. [Content Brief]

[5]. Lee D, et al. Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children. Science. 2023 Feb 10;379(6632):eabo3627. [Content Brief]

[6]. Kirby CS, et al. RNase L represses hair follicle regeneration through altered innate immune signaling. J Clin Invest. 2025 Feb 4;135(6):e172595. [Content Brief]

[7]. Daou S, et al. A phenolic small molecule inhibitor of RNase L prevents cell death from ADAR1 deficiency. Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):24802-24812. [Content Brief]

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RNase L Antibody (YA2314) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20ug) and THP-1(lane 3(40ug) using RNase L Antibody (HY-P82569) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2, 20μg), THP-1 (lane3, 20μg), K562 (lane4, 20μg) and HCT116 (lane5, 20μg) using RNase L Antibody (HY-P82569). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HL-60 (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using RNase L Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RNase L Antibody (YA2314) Product Image
    1/16
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • RNase L Antibody (YA2314) Product Image
  • Experimental Validation Results for RNase L Antibody (YA2314)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20ug) and THP-1(lane 3(40ug) using RNase L Antibody (HY-P82569) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RNase L Antibody (YA2314)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2, 20μg), THP-1 (lane3, 20μg), K562 (lane4, 20μg) and HCT116 (lane5, 20μg) using RNase L Antibody (HY-P82569). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RNase L Antibody (YA2314)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HL-60 (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using RNase L Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • Experimental Validation Results for RNase L Antibody (YA2314)
  • RNase L Antibody (YA2314) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from THP-1(lane 2(20ug) and THP-1(lane 3(40ug) using RNase L Antibody (HY-P82569) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RNase L Antibody (YA2314) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane2, 20μg), THP-1 (lane3, 20μg), K562 (lane4, 20μg) and HCT116 (lane5, 20μg) using RNase L Antibody (HY-P82569). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • RNase L Antibody (YA2314) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HL-60 (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using RNase L Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RNase L Antibody (YA2314) Product Image WB
  • RNase L Antibody (YA2314) Product Image WB
  • RNase L Antibody (YA2314) Product Image WB