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6X His-tag (C-terminal) Antibody (YA860)

(Synonyms: his-tag)
Customer Review

Based on 1 Customer Validation

Anti-6X His-tag (C-terminal) Antibody is a non-conjugated and Mouse origined IgG2a monoclonal antibody, targeting to 6X His-tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for 6X His-tag (C-terminal) Antibody (YA860)
  • 6X His-tag (C-terminal) Antibody (YA860) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000-1:20000 Use at an assay dependent concentration.

Product Details

Description

Anti-6X His-tag (C-terminal) Antibody is a non-conjugated and Mouse origined IgG2a monoclonal antibody, targeting to 6X His-tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Immunogen

Synthetic peptide corresponding to His-tag.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3102764

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for 6X His-tag (C-terminal) Antibody (YA860)
    Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) (HY-P80947) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    6X His-tag is a Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni2+) or cobalt (Co2+), making it useful for protein purification.

  • Synonyms

    his-tag

6X His-tag (C-terminal) Antibody (YA860) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) (HY-P80947) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 6X His-tag (C-terminal) Antibody (YA860) Product Image
    1/16
  • Experimental Validation Results for 6X His-tag (C-terminal) Antibody (YA860)
  • 6X His-tag (C-terminal) Antibody (YA860) Product Image
  • Experimental Validation Results for 6X His-tag (C-terminal) Antibody (YA860)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) (HY-P80947) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for 6X His-tag (C-terminal) Antibody (YA860)
  • 6X His-tag (C-terminal) Antibody (YA860) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) (HY-P80947) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 6X His-tag (C-terminal) Antibody (YA860) Product Image WB