Desmoplakin Antibody (YA2379)(PBS only)

Cat. No.: HY-P82634A: Data Sheet User Guide for Antibodies
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Desmoplakin Antibody (YA2379) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Desmoplakin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Desmoplakin Antibody (YA2379) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Desmoplakin.

Host

Rabbit

Molecular Weight

Predicted band size: 332 kDa;

Observed band size: 270 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P15924

Gene ID

1832 [NCBI]

Immunogen

A synthesized peptide derived from human Desmoplakin

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:500-1:1000
FC FC: Flow Cytometry	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity)

Subcellular Localization：Cell junction, desmosome; Cell membrane; Cytoplasm

Expression：
Tissue_specificity:Expressed in oral mucosa (at protein level) (PubMed:30479852) . Expressed in arrector pili muscle (at protein level) (PubMed:29034528) . Expressed in the heart in the heart (at protein level) (PubMed:18662195) ; Apparently an obligate constituent of all desmosomes; Resides predominantly in tissues and cells of stratified origin

Isoforms & Post-Translational Modification：P15924 has 3 isomers: P15924-1: 331774 Da (predicted); P15924-2: 260119 Da (predicted); P15924-3: 278916 Da (predicted).
Phosphorylation at Ser-2849 increases association with intermediate filament cytokeratin, potentially facilitating interaction between desmosome junctions and intermediate filament architecture

Subunit：Homodimer. Interacts with COL17A1 (via cytoplasmic region) (PubMed:12482924). Interacts with DSC2 (PubMed:21062920). Interacts with PKP2 (PubMed:11790773, PubMed:22781308). Interacts with PKP1 (PubMed:10852826, PubMed:11790773, PubMed:23444369). Interacts weakly with TMEM65

Research Field

Signal Transduction

Synonyms

Desmoplakin I; Desmoplakin II; DPI; DPII; DSP; KPPS2; PPKS2

Documentation

User Guide for Antibodies (1077 KB)