2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. BRCA1 Antibody (YA7459)

BRCA1 Antibody (YA7459) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRCA1.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

BRCA1 Antibody (YA7459) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRCA1.
Host

Rabbit
Clonality

Monoclonal,Recombinant
分子量
Predicted band size: 208 kDa;
Observed band size: 270 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Immunogen

The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:500
WB
WB: Western Blot
1:10000-1:50000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-1:20000
IP
IP: Immunoprecipitation
1:50-1:200
Sensitivity Endogenous 純度 Protein A affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG,Kappa  
Appearance

Liquid
Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:10500182, PubMed:12887909, PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172). Regulates centrosomal microtubule nucleation (PubMed:18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:11836499, PubMed:12183412, PubMed:19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698). Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks (PubMed:19369211). Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8 (PubMed:16818604). Acts as a transcriptional activator (PubMed:20160719)
Subcellular Localization：Nucleus,Chromosome,Cytoplasm,Cytoplasm,Cytoplasm
Expression：
Tissue_Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines
Isoforms & Post-Translational Modification：P38398 has 8 isomers: P38398-1: 207721 Da (predicted); P38398-2: 7177 Da (predicted); P38398-3: 85001 Da (predicted); P38398-4: 205838 Da (predicted); P38398-5: 80590 Da (predicted); P38398-6: 78165 Da (predicted); P38398-7: 210266 Da (predicted); P38398-8: 202342 Da (predicted).
Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR (PubMed:11114888, PubMed:12183412, PubMed:21144835)丨Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination
Subunit：Heterodimer with BARD1 (PubMed:11573085, PubMed:12890688, PubMed:14976165)
ドキュメンテーション
User Guide for Antibodies (1077 KB)

BRCA1 Antibody (YA7459) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
BRCA1 Antibody (YA7459)
製品番号:
HY-P87774
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