BRCA1 Antibody (YA7459)
BRCA1 Antibody (YA7459) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRCA1.
-
Host:
Rabbit
-
Isotype:
IgG,Kappa
-
Application:
WB, IHC-P, ICC/IF, IP, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:100-1:500 | 1:10000-1:50000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
BRCA1 Antibody (YA7459) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRCA1.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 270 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 208 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Required for FANCD2 targeting to sites of DNA damage. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].
-
Subcellular Localization
Nucleus,Chromosome,Cytoplasm,Cytoplasm,Cytoplasm
-
Expression
Tissue_Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines -
Isoforms & Post-Translational Modification
P38398 has 8 isomers: P38398-1: 207721 Da (predicted); P38398-2: 7177 Da (predicted); P38398-3: 85001 Da (predicted); P38398-4: 205838 Da (predicted); P38398-5: 80590 Da (predicted); P38398-6: 78165 Da (predicted); P38398-7: 210266 Da (predicted); P38398-8: 202342 Da (predicted).
Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR (PubMed:11114888, PubMed:12183412, PubMed:21144835)丨Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination -
Subunit
Heterodimer with BARD1 (PubMed:11573085, PubMed:12890688, PubMed:14976165)
-
SwissProt ID
Documentation
Powered by Bioz
