Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194)

製品番号: HY-P80819: データシート SDS COA User Guide for Antibodies
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Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-GSK3 (Tyr216/Tyr279).

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容量	価格（税別）	在庫状況	数量
10 μL	$94	在庫あり	Estimated Time of Arrival: December 31
50 μL	$249	在庫あり	Estimated Time of Arrival: December 31
100 μL	$405	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•Int Immunopharmacol. 2025 May 7:157:114796. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-GSK3 (Tyr216/Tyr279).

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 51 kDa;

Observed band size: 47-51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat

SwissProt ID

P49840 / P49841

Gene ID

2931 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr216 of Human GSK3 alpha.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：P49840: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2) , CTNNB1/beta-catenin, APC and AXIN1 (PubMed:11749387, PubMed:17478001, PubMed:19366350). Requires primed phosphorylation of the majority of its substrates (PubMed:11749387, PubMed:17478001, PubMed:19366350). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:11749387, PubMed:17478001, PubMed:19366350).
P49841: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2) , EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 (PubMed:11430833, PubMed:12554650, PubMed:14690523, PubMed:16484495, PubMed:1846781, PubMed:20937854, PubMed:9072970). Requires primed phosphorylation of the majority of its substrates (PubMed:11430833, PubMed:16484495). In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:8397507). May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed:8397507). Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase (PubMed:8397507). In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes (PubMed:12554650).

Subcellular Localization：P49841: Cytoplasm; Nucleus; Cell membrane

Expression：
Tissue_specificity: P49841: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain

Isoforms & Post-Translational Modification：P49841 has 2 isomers: P49841-1: 46744 Da (predicted); P49841-2: 48034 Da (predicted).
Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and deactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 (PubMed:25169422). Inactivated by phosphorylation at Ser-9 (Probable). Phosphorylated in a circadian manner in the hippocampus (By similarity);Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity;Palmitoylated. Palmitoylation by ZDHHC4 prevents AKT1-mediated phosphorylation

Subunit：P49840: Monomer. Interacts with ARRB2 (By similarity). Interacts with AXIN1 and CTNNB1/beta-catenin (PubMed:17229088).
P49841: Monomer. Interacts with ARRB2, DISC1 and ZBED3 (By similarity).

RRID

AB_3102209

Database

Entrez Gene: 2931 Human ; 2932 Human

SwissProt: P49840 Human ; P49841 Human

Research Field

Neuroscience

Synonyms

Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

ドキュメンテーション

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データシート (235 KB) SDS (252 KB)

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