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DDIT3 Antibody (YA3507)

(Synonyms: GADD153; CHOP; Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.)
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Based on 1 publication(s) in Google Scholar

DDIT3 Antibody is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DDIT3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • DDIT3 Antibody (YA3507) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

DDIT3 Antibody is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DDIT3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19kDa
Species Reactivity Database
Immunogen

Synthetic peptide conjugated to KLH.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103287

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    Western blot analysis of extracts from C6(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH/3T3(lane 4(20μg)and K562( lane 5(20μg) using DDIT3 Antibody (HY-P81233). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed:15322075, PubMed:15775988, PubMed:19672300). Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed:15322075, PubMed:15775988). Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (By similarity). Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (By similarity). Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L (PubMed:15775988, PubMed:17709599, PubMed:20876114, PubMed:22761832). Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) (PubMed:18940792, PubMed:19672300, PubMed:20829347). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress (By similarity). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity (PubMed:16434966). Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response (By similarity). Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling (By similarity)

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Induction:Up-regulated by oxidative stress, amino-acid deprivation, hypoxia and endoplasmic reticulum stress (PubMed:33384352) . During ER stress, induced by a EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during prolonged ER stress

  • Isoforms & Post-Translational Modification

    P35638 has 3 isomers: P35638-1: 19175 Da (predicted); P35638-2: 21700 Da (predicted); P0DPQ6-1: 4284 Da (predicted).
    Ubiquitinated, leading to its degradation by the proteasome;Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity

  • Subunit

    Heterodimer (PubMed:29083303). Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6 (PubMed:16434966, PubMed:17872950). Interacts with TRIB3 which blocks its association with EP300/P300 (PubMed:15775988, PubMed:17872950). Interacts with FOXO3, CEBPB and ATF4 (PubMed:18940792, PubMed:20829347, PubMed:22761832)

  • SwissProt ID

    P35638

  • Gene ID
    1649 [NCBI]
  • Synonyms

    GADD153; CHOP; Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.

DDIT3 Antibody (YA3507) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH/3T3(lane 4(20μg)and K562( lane 5(20μg) using DDIT3 Antibody (HY-P81233). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • DDIT3 Antibody (YA3507) Product Image
    1/16
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • DDIT3 Antibody (YA3507) Product Image
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH/3T3(lane 4(20μg)and K562( lane 5(20μg) using DDIT3 Antibody (HY-P81233). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • Experimental Validation Results for DDIT3 Antibody (YA3507)
  • DDIT3 Antibody (YA3507) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20μg) , HepG2(lane 3(20μg) ,NIH/3T3(lane 4(20μg)and K562( lane 5(20μg) using DDIT3 Antibody (HY-P81233). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • DDIT3 Antibody (YA3507) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • DDIT3 Antibody (YA3507) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • DDIT3 Antibody (YA3507) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • DDIT3 Antibody (YA3507) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human esophageal cancer tissue using DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81233, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • DDIT3 Antibody (YA3507) Product Image WB
  • DDIT3 Antibody (YA3507) Product Image IHC-P
  • DDIT3 Antibody (YA3507) Product Image IHC-P
  • DDIT3 Antibody (YA3507) Product Image mIHC
  • DDIT3 Antibody (YA3507) Product Image mIHC