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Phospho-EIF2S1 (Ser51) Antibody (YA203)

(Synonyms: EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha)
4 Cited Publications
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Based on 4 publication(s) in Google Scholar

Phospho-EIF2S1 (Ser51) Antibody (YA203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-EIF2S1 (Ser51).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
    2.Supplied in PBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-EIF2S1 (Ser51) Antibody (YA203)
  • Phospho-EIF2S1 (Ser51) Antibody (YA203) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Phospho-EIF2S1 (Ser51) Antibody (YA203)

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:10000 1:200-1:800

Product Details

Description

Phospho-EIF2S1 (Ser51) Antibody (YA203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-EIF2S1 (Ser51).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser51 of Human EIF2S1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102203

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
    2.Supplied in PBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-EIF2S1 (Ser51) Antibody (YA203)
    Western blot analysis of extracts from K-562(lane 2(20μg) ,C6(lane 3(20μg) and NIH/3T3(lane 4(20ug)using Phospho-eIF2A Antibody (HY-P80811) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    EIF2S1 is a Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B. EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, Stress granule; Cytoplasm, cytosol; Mitochondrion

  • Expression


    Induction:Up-regulated upon endoplasmic reticulum stress

  • Subunit

    Eukaryotic translation initiation factor 2 eIF2 is a heterotrimeric complex composed of an alpha (EIF2S1), a beta (EIF2S2) and a gamma (EIF2S3) chain (PubMed:23063529, PubMed:31048492, PubMed:31836389, PubMed:35031321). eIF2 is member of the 43S pre-initiation complex (43S PIC). eIF2 forms a complex with at least CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Interacts with ABCF1 isoform 2 (PubMed:17894550). Associates with ribosomes (PubMed:17894550). Interacts with DDX3X in an RNA-independent manner (PubMed:18596238). Interacts with CDC123 (PubMed:31836389, PubMed:35031321)

  • SwissProt ID

    P05198

  • Gene ID
    1965 [NCBI]
  • Synonyms

    EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Mikami S, et al. An efficient mammalian cell-free translation system supplemented with translation factors. Protein Expr Purif. 2006 Apr;46(2):348-57. [Content Brief]

[2]. Chakrabarty Y, et al. The HRI branch of the integrated stress response selectively triggers mitophagy. Mol Cell. 2024 Mar 21;84(6):1090-1100.e6. [Content Brief]

[3]. Lee YY, et al. An upstream open reading frame regulates translation of GADD34 during cellular stresses that induce eIF2alpha phosphorylation. J Biol Chem. 2009 Mar 13;284(11):6661-73. [Content Brief]

[4]. You K, et al. QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis. Science. 2021 Jan 1;371(6524):. [Content Brief]

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Phospho-EIF2S1 (Ser51) Antibody (YA203) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K-562(lane 2(20μg) ,C6(lane 3(20μg) and NIH/3T3(lane 4(20ug)using Phospho-eIF2A Antibody (HY-P80811) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-EIF2S1 (Ser51) Antibody (YA203) Product Image
    1/16
  • Experimental Validation Results for Phospho-EIF2S1 (Ser51) Antibody (YA203)
  • Phospho-EIF2S1 (Ser51) Antibody (YA203) Product Image
  • Experimental Validation Results for Phospho-EIF2S1 (Ser51) Antibody (YA203)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K-562(lane 2(20μg) ,C6(lane 3(20μg) and NIH/3T3(lane 4(20ug)using Phospho-eIF2A Antibody (HY-P80811) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-EIF2S1 (Ser51) Antibody (YA203)
  • Phospho-EIF2S1 (Ser51) Antibody (YA203) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K-562(lane 2(20μg) ,C6(lane 3(20μg) and NIH/3T3(lane 4(20ug)using Phospho-eIF2A Antibody (HY-P80811) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-EIF2S1 (Ser51) Antibody (YA203) Product Image WB