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Calpastatin Antibody (YA814)

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Based on 1 Customer Validation

Calpastatin Antibody (YA814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Calpastatin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Calpastatin Antibody (YA814)
  • Calpastatin Antibody (YA814) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500 1:100 1:200

Product Details

Description

Calpastatin Antibody (YA814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Calpastatin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 76.5 kDa
Species Reactivity Database

Entrez Gene: 831 Human

SwissProt: P20810 Human

Immunogen

Synthetic peptide corresponding to Human Calpastatin.AA range:501-708.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Calpastatin Antibody (YA814)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) and A549 (lane 2(20μg)) using Calpastatin Antibody (HY-P80042) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Calpastatin is a Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue

  • Isoforms & Post-Translational Modification

    P20810 has 10 isomers: P20810-1: 76573 Da (predicted); P20810-2: 75304 Da (predicted); P20810-3: 63665 Da (predicted); P20810-4: 71894 Da (predicted); P20810-5: 81169 Da (predicted); P20810-6: 84943 Da (predicted); P20810-7: 82771 Da (predicted); P20810-8: 74067 Da (predicted); P20810-9: 80265 Da (predicted); P20810-10: 82437 Da (predicted).
    The N-terminus is blocked

  • SwissProt ID

    P20810

  • Gene ID
    831 [NCBI]
  • Research Field

    Neuroscience

Calpastatin Antibody (YA814) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) and A549 (lane 2(20μg)) using Calpastatin Antibody (HY-P80042) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Calpastatin Antibody (YA814) Product Image
    1/16
  • Experimental Validation Results for Calpastatin Antibody (YA814)
  • Calpastatin Antibody (YA814) Product Image
  • Experimental Validation Results for Calpastatin Antibody (YA814)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) and A549 (lane 2(20μg)) using Calpastatin Antibody (HY-P80042) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Calpastatin Antibody (YA814)
  • Calpastatin Antibody (YA814) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) and A549 (lane 2(20μg)) using Calpastatin Antibody (HY-P80042) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Calpastatin Antibody (YA814) Product Image WB