Acetyl Coenzyme A Carboxylase Antibody (YA6016)

Cat. No.: HY-P86324: Data Sheet COA
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Acetyl Coenzyme A Carboxylase Antibody (YA6016) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Acetyl Coenzyme A Carboxylase Antibody (YA6016) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 227 kDa;

Observed band size: 265 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O00763

Gene ID

32 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:4000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG, IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C2C12 (lane 4(20μg) and C6 (lane 5(20μg) using Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed:16854592, PubMed:19236960, PubMed:19900410, PubMed:20457939, PubMed:20952656, PubMed:26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:19236960, PubMed:20457939, PubMed:20952656, PubMed:26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity)

Subcellular Localization：Mitochondrion

Expression：
Tissue_specificity:This gene is widely expressed in the heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland, and colon, with the highest expression levels found in these areas (PubMed: 9099716) . Isomer 3 is expressed in skeletal muscle, adipose tissue, and liver (protein level) (PubMed: 19190759) . Isomer 3 is present in higher amounts in adipose tissue and in lower amounts in the heart, liver, skeletal muscle, and testes (PubMed: 19190759) .

Isoforms & Post-Translational Modification：O00763 has 3 isomers: O00763-1: 276541 Da (predicted); O00763-2: 268166 Da (predicted); O00763-3: 255093 Da (predicted).
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity;Phosphorylation at Ser-222 by AMPK inactivates the enzyme (PubMed:12488245). Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity)

Subunit：Monomer, homodimer, and homotetramer (PubMed:18772397, PubMed:20952656). Forms filamentous polymers (PubMed:19900410, PubMed:20457939, PubMed:20952656). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity in a citrate-dependent manner (PubMed:20457939, PubMed:20952656)

Synonyms

ACACB,Acetyl CoA carboxylase 2,ACC beta,ACC2,ACCB,AcetylCoA carboxylase 2,ACCbeta,ACCβ,ACC β,

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)