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Acetyl Coenzyme A Carboxylase Antibody (YA6016)

(Synonyms: ACACB,Acetyl CoA carboxylase 2,ACC beta,ACC2,ACCB,AcetylCoA carboxylase 2,ACCbeta,ACCβ,ACC β,)
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Based on 1 Customer Validation

Acetyl Coenzyme A Carboxylase Antibody (YA6016) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:4000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Acetyl Coenzyme A Carboxylase Antibody (YA6016) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 265 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 227 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C2C12 (lane 4(20μg) and C6 (lane 5(20μg) using Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Acetyl Coenzyme A Carboxylase is a Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation. Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage[1][2][3][4][5][6].

  • Subcellular Localization

    Mitochondrion

  • Expression


    Tissue_specificity:This gene is widely expressed in the heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland, and colon, with the highest expression levels found in these areas (PubMed: 9099716) . Isomer 3 is expressed in skeletal muscle, adipose tissue, and liver (protein level) (PubMed: 19190759) . Isomer 3 is present in higher amounts in adipose tissue and in lower amounts in the heart, liver, skeletal muscle, and testes (PubMed: 19190759) .

  • Isoforms & Post-Translational Modification

    O00763 has 3 isomers: O00763-1: 276541 Da (predicted); O00763-2: 268166 Da (predicted); O00763-3: 255093 Da (predicted).
    The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity;Phosphorylation at Ser-222 by AMPK inactivates the enzyme (PubMed:12488245). Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity)

  • Subunit

    Monomer, homodimer, and homotetramer (PubMed:18772397, PubMed:20952656). Forms filamentous polymers (PubMed:19900410, PubMed:20457939, PubMed:20952656). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity in a citrate-dependent manner (PubMed:20457939, PubMed:20952656)

  • SwissProt ID

    O00763

  • Gene ID
    32 [NCBI]
  • Synonyms

    ACACB,Acetyl CoA carboxylase 2,ACC beta,ACC2,ACCB,AcetylCoA carboxylase 2,ACCbeta,ACCβ,ACC β,

Acetyl Coenzyme A Carboxylase Antibody (YA6016) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C2C12 (lane 4(20μg) and C6 (lane 5(20μg) using Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
    1/16
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C2C12 (lane 4(20μg) and C6 (lane 5(20μg) using Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Experimental Validation Results for Acetyl Coenzyme A Carboxylase Antibody (YA6016)
  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), C2C12 (lane 4(20μg) and C6 (lane 5(20μg) using Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Acetyl Coenzyme A Carboxylase with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Acetyl Coenzyme A Carboxylase Antibody (HY-P86324) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image WB
  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image ICC
  • Acetyl Coenzyme A Carboxylase Antibody (YA6016) Product Image ICC