Phospho-BCKDHA (S293) Antibody (YA6882)

Cat. No.: HY-P87194: Data Sheet COA
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Phospho-BCKDHA (S293) Antibody (YA6882) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-BCKDHA (S293).

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

Phospho-BCKDHA (S293) Antibody (YA6882) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-BCKDHA (S293).

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size：50 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P12694

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human BCKDHA.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:20000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Phosphorylated	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Phospho-BCKDHA (S293) Antibody (HY-P87194) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/20000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction

Subcellular Localization：Mitochondrion matrix

Isoforms & Post-Translational Modification：P12694 has two isomers: P12694-1: 50471 Da (predicted); P12694-2: 50790 Da (predicted).
Phosphorylated at Ser-337 by BCKDK and dephosphorylated by protein phosphatase PPM1K

Subunit：Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex (PubMed:10745006, PubMed:9582350)

Synonyms

Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody

각종 서류

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)