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Phospho-BCKDHA (S293) Antibody (YA6882)

(Synonyms: Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody)
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Based on 1 Customer Validation

Phospho-BCKDHA (S293) Antibody (YA6882) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-BCKDHA (S293).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-BCKDHA (S293) Antibody (YA6882)
  • Phospho-BCKDHA (S293) Antibody (YA6882) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:20000

Product Details

Description

Phospho-BCKDHA (S293) Antibody (YA6882) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-BCKDHA (S293).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：50 kDa
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human BCKDHA.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-BCKDHA (S293) Antibody (YA6882)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Phospho-BCKDHA (S293) Antibody (HY-P87194) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/20000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    BCKDHA is a Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    P12694 has two isomers: P12694-1: 50471 Da (predicted); P12694-2: 50790 Da (predicted).
    Phosphorylated at Ser-337 by BCKDK and dephosphorylated by protein phosphatase PPM1K

  • Subunit

    Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex (PubMed:10745006, PubMed:9582350)

  • SwissProt ID

    P12694

  • Synonyms

    Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody

Phospho-BCKDHA (S293) Antibody (YA6882) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Phospho-BCKDHA (S293) Antibody (HY-P87194) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/20000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Phospho-BCKDHA (S293) Antibody (YA6882) Product Image
    1/16
  • Experimental Validation Results for Phospho-BCKDHA (S293) Antibody (YA6882)
  • Phospho-BCKDHA (S293) Antibody (YA6882) Product Image
  • Experimental Validation Results for Phospho-BCKDHA (S293) Antibody (YA6882)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Phospho-BCKDHA (S293) Antibody (HY-P87194) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/20000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-BCKDHA (S293) Antibody (YA6882)
  • Phospho-BCKDHA (S293) Antibody (YA6882) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Phospho-BCKDHA (S293) Antibody (HY-P87194) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/20000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Phospho-BCKDHA (S293) Antibody (YA6882) Product Image WB