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CD223/LAG-3 Antibody (YA3842)

(Synonyms: LAG3)
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Based on 1 Customer Validation

CD223/LAG-3 Antibody (YA3842) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD223/LAG-3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • CD223/LAG-3 Antibody (YA3842) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

CD223/LAG-3 Antibody (YA3842) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD223/LAG-3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Purified recombinant fragment of human CD223 (AA: extra 66-199) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
    Western blot analysis was performed on protein extracts (40 μg) from A549 (lane 2), SH-SY5Y (lane 3), HT-1080 (lane 4), and U87 (lane 5) using CD223/LAG-3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
    Western blot analysis of extracts from Jurkat (lane 2, 20ug), K562 (lane 3, 20ug), A549 (lane 4, 20ug) and Hela (lane 5, 20ug) using CD223/LAG-3 Antibody (HY-P84145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD223/LAG-3 is a Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear; May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:It is mainly expressed in activated T cells and some natural killer (NK) cells.

    Induction:Expression is induced by interleukin-2 (IL2) , interleukin-7 (IL7) and interleukin-12 (IL12A and IL12B) on activated T-cells

  • Subunit

    Interacts with MHC class II (MHC-II); selectively recognizes stable complexes of peptide and MHC-II (PubMed:1692078, PubMed:7589152, PubMed:8647185, PubMed:9159144). Interacts with FGL1 (via the Fibrinogen C-terminal domain) (PubMed:30580966)

  • SwissProt ID

    P18627

  • Gene ID
    3902 [NCBI]
  • Synonyms

    LAG3

[1]. Camisaschi C, et al. LAG-3 expression defines a subset of CD4(+)CD25(high)Foxp3(+) regulatory T cells that are expanded at tumor sites. J Immunol. 2010 Jun 1;184(11):6545-51. [Content Brief]

[2]. Huard B, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol. 1994 Dec;24(12):3216-21. [Content Brief]

[3]. Huard B, et al. T cell major histocompatibility complex class II molecules down-regulate CD4+ T cell clone responses following LAG-3 binding. Eur J Immunol. 1996 May;26(5):1180-6. [Content Brief]

CD223/LAG-3 Antibody (YA3842) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from A549 (lane 2), SH-SY5Y (lane 3), HT-1080 (lane 4), and U87 (lane 5) using CD223/LAG-3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 20ug), K562 (lane 3, 20ug), A549 (lane 4, 20ug) and Hela (lane 5, 20ug) using CD223/LAG-3 Antibody (HY-P84145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD223/LAG-3 Antibody (YA3842) Product Image
    1/16
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • CD223/LAG-3 Antibody (YA3842) Product Image
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from A549 (lane 2), SH-SY5Y (lane 3), HT-1080 (lane 4), and U87 (lane 5) using CD223/LAG-3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 20ug), K562 (lane 3, 20ug), A549 (lane 4, 20ug) and Hela (lane 5, 20ug) using CD223/LAG-3 Antibody (HY-P84145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • Experimental Validation Results for CD223/LAG-3 Antibody (YA3842)
  • CD223/LAG-3 Antibody (YA3842) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (40 μg) from A549 (lane 2), SH-SY5Y (lane 3), HT-1080 (lane 4), and U87 (lane 5) using CD223/LAG-3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD223/LAG-3 Antibody (YA3842) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2, 20ug), K562 (lane 3, 20ug), A549 (lane 4, 20ug) and Hela (lane 5, 20ug) using CD223/LAG-3 Antibody (HY-P84145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD223/LAG-3 Antibody (YA3842) Product Image WB
  • CD223/LAG-3 Antibody (YA3842) Product Image WB