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GABA A Receptor beta 1 Antibody (YA2261)

(Synonyms: GABA-A receptor; beta-1 polypeptide; Gabrb-1; GABRB1; GARB1)
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Based on 1 Customer Validation

GABA A Receptor beta 1 Antibody (YA2261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GABA A Receptor beta 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GABA A Receptor beta 1 Antibody (YA2261)
  • GABA A Receptor beta 1 Antibody (YA2261) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

GABA A Receptor beta 1 Antibody (YA2261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GABA A Receptor beta 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GABA A Receptor beta 1 aa371-400.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GABA A Receptor beta 1 Antibody (YA2261)
    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using GABA A Receptor beta 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    GABA A Receptor beta 1 is a Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain one or two GABA active binding sites located at the alpha and beta subunit interfaces, depending on subunit composition. When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness[1][2][3].

  • Subcellular Localization

    Postsynaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P18505 has 2 isomers: P18505-1: 54235 Da (predicted); P18505-2: 10151 Da (predicted).

  • Subunit

    Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties (PubMed:10449790, PubMed:16412217). Binds UBQLN1 (By similarity)

  • SwissProt ID

    P18505

  • Gene ID
    2560 [NCBI]
  • Synonyms

    GABA-A receptor; beta-1 polypeptide; Gabrb-1; GABRB1; GARB1

  • Research Field

    Neuroscience

References

[1]. Bonnert TP, et al. theta, a novel gamma-aminobutyric acid type A receptor subunit. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9891-6. [Content Brief]

[2]. Ranna M, et al. Impact of epsilon and theta subunits on pharmacological properties of alpha3beta1 GABAA receptors expressed in Xenopus oocytes. BMC Pharmacol. 2006 Jan 13;6:1. [Content Brief]

[3]. Janve VS, et al. Epileptic encephalopathy de novo GABRB mutations impair γ-aminobutyric acid type A receptor function. Ann Neurol. 2016 May;79(5):806-825. [Content Brief]

GABA A Receptor beta 1 Antibody (YA2261) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using GABA A Receptor beta 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GABA A Receptor beta 1 Antibody (YA2261) Product Image
    1/16
  • Experimental Validation Results for GABA A Receptor beta 1 Antibody (YA2261)
  • GABA A Receptor beta 1 Antibody (YA2261) Product Image
  • Experimental Validation Results for GABA A Receptor beta 1 Antibody (YA2261)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using GABA A Receptor beta 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GABA A Receptor beta 1 Antibody (YA2261)
  • GABA A Receptor beta 1 Antibody (YA2261) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Mouse brain (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using GABA A Receptor beta 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GABA A Receptor beta 1 Antibody (YA2261) Product Image WB