2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Flow Cytometry Antibodies
  5. Protein Phosphatase 4C Antibody (YA5150)

Protein Phosphatase 4C Antibody (YA5150)

製品番号: HY-P85458
User Guide for Antibodies Technical Support

Protein Phosphatase 4C Antibody (YA5150) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Protein Phosphatase 4C.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Protein Phosphatase 4C Antibody (YA5150) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Protein Phosphatase 4C.
Host

Rabbit
Clonality

Monoclonal
分子量

Predicted band size: 35 kDa;

Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Purified recombinant human PPX protein fragments expressed in E.coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
FC
FC: Flow Cytometry
1:200-1:500
Sensitivity Endogenous 純度 affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin
Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Subunit：Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 (PubMed:12668731). Interacts with REL, NFKB1/p50 and RELA (PubMed:1336397). Interacts with SMN1 and GEMIN4 (PubMed:12668731). Interacts with IRS4 (phosphorylated) (PubMed:15331607). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 (PubMed:16085932). Interacts with HDAC3 (PubMed:15805470)
Synonyms
PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-X
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Protein Phosphatase 4C Antibody (YA5150) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Protein Phosphatase 4C Antibody (YA5150)
製品番号:
HY-P85458
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