Protein Phosphatase 4C Antibody (YA5150)
(Synonyms: PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-X)Protein Phosphatase 4C Antibody (YA5150) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Protein Phosphatase 4C.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:200-1:500 |
Product Details
Protein Phosphatase 4C Antibody (YA5150) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Protein Phosphatase 4C.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 35 kDa;
Purified recombinant human PPX protein fragments expressed in E.coli.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Protein Phosphatase 4C is a Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin
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Subcellular Localization
Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Subunit
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 (PubMed:12668731). Interacts with REL, NFKB1/p50 and RELA (PubMed:1336397). Interacts with SMN1 and GEMIN4 (PubMed:12668731). Interacts with IRS4 (phosphorylated) (PubMed:15331607). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 (PubMed:16085932). Interacts with HDAC3 (PubMed:15805470)
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SwissProt ID
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Synonyms
PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-X
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