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BACH1 Antibody (YA2061)

(Synonyms: BACH 1; BRIP 1; BRIP1; FANCJ)
Customer Review

Based on 1 Customer Validation

BACH1 Antibody (YA2061) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BACH1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • BACH1 Antibody (YA2061) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

BACH1 Antibody (YA2061) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BACH1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 105 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 141 kDa
Species Reactivity Database

Entrez Gene: 83990 Human

SwissProt: Q9BX63 Human

Immunogen

A synthesized peptide derived from human BACH1/BRIP1 aa400-450/1249.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104427

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BACH1 Antibody (YA2061)
    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293T (lane 3, 15 μg), and A431 (lane 4, 15 μg) using BRIP1(N-terminal) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for BACH1 Antibody (YA2061)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using BACH1 Antibody (HY-P82316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    BACH1 is a DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease. Can unwind RNA:DNA substrates. Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:It is widely expressed, with the highest expression level in the testes.

  • Isoforms & Post-Translational Modification

    Q9BX63 has 2 isomers: Q9BX63-1: 140867 Da (predicted); Q9BX63-2: 112466 Da (predicted).
    Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated;Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling

  • Subunit

    Interacts with the replication protein A complex (RPA) via the RPA1 subunit; following DNA damage they colocalize in foci in the nucleus (PubMed:17596542). Binds directly to the BRCT domains of BRCA1 (PubMed:15125843). Interacts with the CIA complex components CIAO1, CIAO2B and MMS19 (PubMed:23585563)

  • SwissProt ID

    Q9BX63

  • Gene ID
    83990 [NCBI]
  • Synonyms

    BACH 1; BRIP 1; BRIP1; FANCJ

  • Research Field

    -

BACH1 Antibody (YA2061) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293T (lane 3, 15 μg), and A431 (lane 4, 15 μg) using BRIP1(N-terminal) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using BACH1 Antibody (HY-P82316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • BACH1 Antibody (YA2061) Product Image
    1/16
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • BACH1 Antibody (YA2061) Product Image
  • Experimental Validation Results for BACH1 Antibody (YA2061)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293T (lane 3, 15 μg), and A431 (lane 4, 15 μg) using BRIP1(N-terminal) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for BACH1 Antibody (YA2061)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using BACH1 Antibody (HY-P82316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • Experimental Validation Results for BACH1 Antibody (YA2061)
  • BACH1 Antibody (YA2061) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293T (lane 3, 15 μg), and A431 (lane 4, 15 μg) using BRIP1(N-terminal) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • BACH1 Antibody (YA2061) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using BACH1 Antibody (HY-P82316) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • BACH1 Antibody (YA2061) Product Image WB
  • BACH1 Antibody (YA2061) Product Image WB