ERK5 Antibody (YA5416)

Cat. No.: HY-P85724: Data Sheet User Guide for Antibodies
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ERK5 Antibody (YA5416) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ERK5.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

ERK5 Antibody (YA5416) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ERK5.

Host

Mouse

Clonality

Monoclonal

분자량

Observed band size: 115 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13164

Gene ID

5598 [NCBI]

Immunogen

Synthesized peptide derived from human ERK5

Application &
Dilution Ratio

Application	Dilution Ratio
IHC1	50-200
WB WB: Western Blot	1:1000-2000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction

Subcellular Localization：Cytoplasm; Nucleus; Nucleus, PML body

Expression：
Tissue_specificity:It is expressed in many adult tissues. It is abundant in the heart, placenta, lungs, kidneys, and skeletal muscle. It is undetectable in the liver.

Isoforms & Post-Translational Modification：Q13164 has 4 isomers: Q13164-1: 88386 Da (predicted); Q13164-2: 73218 Da (predicted); Q13164-3: 59328 Da (predicted); Q13164-4: 50152 Da (predicted).
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent

Subunit：Interacts with MAP2K5.

각종 서류

User Guide for Antibodies (1077 KB)