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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Radixin Antibody (YA1810)

Radixin Antibody (YA1810)

Cat. No.: HY-P82065
COA
SDS
User Guide for Antibodies Technical Support

Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Documentation

Description

Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 69 kDa;
Observed band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Radixin aa400-450.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:200
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:20-1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
ALL WB ICC

  • Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), 3T3-L1 (lane 4(20μg), C6 (lane 5(20μg) using Radixin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling Radixin Antibody with Radixin Antibody (YA1810) (HY-P82065) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Radixin Antibody (YA1810) ((HY-P82065) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling Radixin Antibody with Radixin Antibody (YA1810) (HY-P82065) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Radixin Antibody (YA1810) ((HY-P82065) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane
Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Cleavage furrow; Cell projection, microvillus; Cell projection, stereocilium
Isoforms & Post-Translational Modification：P35241 has 5 isomers: P35241-1: 68564 Da (predicted); P35241-2: 29668 Da (predicted); P35241-3: 23413 Da (predicted); P35241-4: 52679 Da (predicted); P35241-5: 71049 Da (predicted).
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity)
Subunit：Binds NHERF1 (PubMed:9430655). Interacts with NHERF1, NHERF2, LAYN, MME/NEP and ICAM2. Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts (via FERM domain) with SPN/CD43 cytoplasmic tail (By similarity). Interacts with CD44 (By similarity). Interacts with CLIC5; may work together in a complex which also includes EZR and MYO6 to stabilize linkages between the plasma membrane and subjacent actin cytoskeleton at the base of stereocilia (By similarity)
Database

Entrez Gene: 5962 Human ; 19684 Mouse ; 315655 Rat

SwissProt: P35241 Human ; P26043 Mouse ;

OMIM: 611022 Human

Research Field

Signal Transduction
Synonyms
RDX; Radixin
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Radixin Antibody (YA1810) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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