Radixin Antibody (YA1810)

Cat. No.: HY-P82065: Data Sheet COA
SDS
User Guide for Antibodies
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Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Radixin Antibody (YA1810) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Radixin Antibody (YA1810) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Radixin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 69 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35241

Gene ID

5962 [NCBI]

Immunogen

A synthetic peptide of human Radixin aa400-450.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:20-1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from K562 (lane 2(20μg), Hela (lane 3(20μg), 3T3-L1 (lane 4(20μg), C6 (lane 5(20μg) using Radixin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Radixin Antibody with Radixin Antibody (YA1810) (HY-P82065) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Radixin Antibody (YA1810) ((HY-P82065) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Radixin Antibody with Radixin Antibody (YA1810) (HY-P82065) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Radixin Antibody (YA1810) ((HY-P82065) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Cleavage furrow; Cell projection, microvillus; Cell projection, stereocilium

Isoforms & Post-Translational Modification：P35241 has 5 isomers: P35241-1: 68564 Da (predicted); P35241-2: 29668 Da (predicted); P35241-3: 23413 Da (predicted); P35241-4: 52679 Da (predicted); P35241-5: 71049 Da (predicted).
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity)

Subunit：Binds NHERF1 (PubMed:9430655). Interacts with NHERF1, NHERF2, LAYN, MME/NEP and ICAM2. Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts (via FERM domain) with SPN/CD43 cytoplasmic tail (By similarity). Interacts with CD44 (By similarity). Interacts with CLIC5; may work together in a complex which also includes EZR and MYO6 to stabilize linkages between the plasma membrane and subjacent actin cytoskeleton at the base of stereocilia (By similarity)

Database

Entrez Gene: 5962 Human ; 19684 Mouse ; 315655 Rat

SwissProt: P35241 Human ; P26043 Mouse ;

OMIM: 611022 Human

Research Field

Signal Transduction

Synonyms

RDX; Radixin

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Radixin Antibody (YA1810) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Radixin Antibody (YA1810)

Radixin Antibody (YA1810) Featured Recommendations:

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Background

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Radixin Antibody (YA1810) Related Classifications

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SAFETY DATA SHEET (SDS)

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