Integrin alpha 6 Antibody (YA5132)

製品番号: HY-P85440: データシート User Guide for Antibodies
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Integrin alpha 6 Antibody (YA5132) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Integrin alpha 6.

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容量		在庫状況
50 μL		お問い合わせ
100 μL		お問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

Integrin alpha 6 Antibody (YA5132) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Integrin alpha 6.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 127 kDa;

Species Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig

SwissProt ID

P23229

Gene ID

3655 [NCBI]

Immunogen

Purified recombinant human Integrin α6 (N-terminus) protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor

Expression：
Tissue_specificity:Integrin α6/β4 is primarily expressed by epithelial cells. The isoform containing the X1 fragment is universally expressed. The isoform containing the X1X2 fragment is expressed in the heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and a few cell lines; they are always co-expressed with the universally present isoform containing the X1 fragment. In some tissues (e.g., salivary glands) , isoforms containing both cytoplasmic fragment A and fragment B can be detected, while in others, only an isoform containing one cytoplasmic fragment is found (fragment A in the epidermis, fragment B in the kidneys) . Processed integrin α6: expressed at low levels in normal prostate tissue, with elevated levels in prostate cancer tissue (protein level) (PubMed: 15023541) .

Isoforms & Post-Translational Modification：P23229 has 8 isomers: P23229-1: 126606 Da (predicted); P23229-2: 119476 Da (predicted); P23229-3: 122053 Da (predicted); P23229-4: 119088 Da (predicted); P23229-5: 121665 Da (predicted); P23229-6: 124029 Da (predicted); P23229-7: 106577 Da (predicted); P23229-9: 122358 Da (predicted).
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand;Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p) (PubMed:11359780, PubMed:15023541, PubMed:17303120). Production of alpha6p enhances prostate cancer cell invasion and migration (PubMed:17303120);Palmitoylation by DHHC3 enhances stability and cell surface expression

Subunit：Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond (By similarity). Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively (By similarity). ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with ADAM9 (By similarity). Interacts with RAB21 (PubMed:16754960). Interacts with MDK (PubMed:15466886). ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth (PubMed:15466886). Interacts with CD82; this interaction down-regulates ITGA6-mediated cell adhesion (PubMed:15557282)

Synonyms

ITGA6; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD antigen CD49f

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/mda-mb-468-cell.html [Content Brief]