Integrin alpha 6 Antibody (YA5132)
(Synonyms: ITGA6; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD antigen CD49f)Integrin alpha 6 Antibody (YA5132) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Integrin alpha 6.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Bovine
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Formulation:
Supplied in PBS(pH7.4) containing 40% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:4000 | 1:100-200 | 1:15-1:100 | 1:10-50 |
Product Details
Integrin alpha 6 Antibody (YA5132) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Integrin alpha 6.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Bovine Predicted Reactivity: Mouse,Rat,Pig,Dog
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Calculated Molecular Weight Predicted band size: 127 kDa;
Purified recombinant protein of human Integrin α6.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 40% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Integrin alpha 6 is an Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling[1][2][3].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor
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Expression
Tissue_specificity:Integrin α6/β4 is primarily expressed by epithelial cells. The isoform containing the X1 fragment is universally expressed. The isoform containing the X1X2 fragment is expressed in the heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and a few cell lines; they are always co-expressed with the universally present isoform containing the X1 fragment. In some tissues (e.g., salivary glands) , isoforms containing both cytoplasmic fragment A and fragment B can be detected, while in others, only an isoform containing one cytoplasmic fragment is found (fragment A in the epidermis, fragment B in the kidneys) . Processed integrin α6: expressed at low levels in normal prostate tissue, with elevated levels in prostate cancer tissue (protein level) (PubMed: 15023541) . -
Isoforms & Post-Translational Modification
P23229 has 8 isomers: P23229-1: 126606 Da (predicted); P23229-2: 119476 Da (predicted); P23229-3: 122053 Da (predicted); P23229-4: 119088 Da (predicted); P23229-5: 121665 Da (predicted); P23229-6: 124029 Da (predicted); P23229-7: 106577 Da (predicted); P23229-9: 122358 Da (predicted).
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand;Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p) (PubMed:11359780, PubMed:15023541, PubMed:17303120). Production of alpha6p enhances prostate cancer cell invasion and migration (PubMed:17303120);Palmitoylation by DHHC3 enhances stability and cell surface expression -
Subunit
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond (By similarity). Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively (By similarity). ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with ADAM9 (By similarity). Interacts with RAB21 (PubMed:16754960). Interacts with MDK (PubMed:15466886). ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth (PubMed:15466886). Interacts with CD82; this interaction down-regulates ITGA6-mediated cell adhesion (PubMed:15557282)
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SwissProt ID
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Synonyms
ITGA6; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD antigen CD49f
Documentation
References
[1]. Ieguchi K, et al. Direct binding of the EGF-like domain of neuregulin-1 to integrins ({alpha}v{beta}3 and {alpha}6{beta}4) is involved in neuregulin-1/ErbB signaling. J Biol Chem. 2010 Oct 8;285(41):31388-98. [Content Brief]
[2]. Fujita M, et al. Cross-talk between integrin α6β4 and insulin-like growth factor-1 receptor (IGF1R) through direct α6β4 binding to IGF1 and subsequent α6β4-IGF1-IGF1R ternary complex formation in anchorage-independent conditions. J Biol Chem. 2012 Apr 6;287(15):12491-500. [Content Brief]
[3]. Cedano Prieto DM, et al. Direct integrin binding to insulin-like growth factor-2 through the C-domain is required for insulin-like growth factor receptor type 1 (IGF1R) signaling. PLoS One. 2017;12(9):e0184285. [Content Brief]
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