Ezrin Antibody (YA3808)(PBS only)

Cat. No.: HY-P84111A: Data Sheet User Guide for Antibodies
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Ezrin Antibody (YA3808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Ezrin Antibody (YA3808) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

Host

Mouse

Molecular Weight

Predicted band size: 69 kDa;

Observed band size: 81 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

P15311

Gene ID

7430 [NCBI]

Immunogen

Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:400
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis

Subcellular Localization：Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cell cortex; Cytoplasm, cytoskeleton; Cell projection, microvillus

Expression：
Tissue_specificity:It is expressed in the cerebral cortex, basal ganglia, hippocampus, pituitary gland, and optic nerve. Expression is weaker in the brainstem and diencephalon. Expression is stronger in the frontal gray matter than in white matter (protein level) . It is a component of the microvilli of intestinal epithelial cells. It is preferentially expressed in astrocytes of the hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Expression was not detected in neurons in most of the tissues studied.

Subunit：Monomer. Homodimer (PubMed:27364155). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, NHERF1 and TMEM8B (PubMed:12651155, PubMed:15498789, PubMed:17616675, PubMed:17881735, PubMed:19555689, PubMed:9314537).

Synonyms

CVL; CVIL; VIL2; HEL-S-105

Documentation

User Guide for Antibodies (1077 KB)